Abstract

Mason-Pfizer Monkey virus (M-PMV) is an exogenous, primate retrovirus that was isolated from a breast carcinoma of a rhesus monkey. It is the prototype virus of the D-type retroviruses which, like the B type retrovirus mouse mammary tumor virus, preassemble a complete capsid within the cytoplasm of a infected cell. These A-type particles migrate to the plasma membrane and are enveloped by a lipid bilayer upon release from the cell. The major goals of this proposal are to define those viral coded gag-gene functions that are essential in assembly, morphogenesis and infectivity of the M-PMV particle. The carboxy-terminal amino acid sequence of the five major gag gene products of this virus will be determined. This information will be used together with amino-terminal amino acid sequence information to define the polypeptide coding regions on the nucleic acid sequence of the gag gene determined from cloned M-PMV DNA. In vitro site directed mutagenesis of the gag gene coding region and of a sequence 3' of gag which may code for the viral protease will be used to determine the function of these regions. Deletion, insertion, and point mutagenesis procedures will be employed for these studies. The role of the envelope glycoproteins in directing the budding and release of viral particales will be investigated utilizing similar procedures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37CA027834-19
Application #
2882298
Study Section
Special Emphasis Panel (NSS)
Program Officer
Read-Connole, Elizabeth Lee
Project Start
1980-09-01
Project End
2000-02-29
Budget Start
1999-03-01
Budget End
2000-02-29
Support Year
19
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Wen, Xiaoyun; Ding, Lingmei; Wang, Jaang-Jiun et al. (2014) ROCK1 and LIM kinase modulate retrovirus particle release and cell-cell transmission events. J Virol 88:6906-21
Wen, Xiaoyun; Ding, Lingmei; Hunter, Eric et al. (2014) An siRNA screen of membrane trafficking genes highlights pathways common to HIV-1 and M-PMV virus assembly and release. PLoS One 9:e106151
Pereira, Lara E; Clark, Jasmine; Grznarova, Petra et al. (2014) Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules. Virology 449:109-19
Prchal, Jan; Srb, Pavel; Hunter, Eric et al. (2012) The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding. J Mol Biol 423:427-38
Hadravova, Romana; de Marco, Alex; Ulbrich, Pavel et al. (2012) In vitro assembly of virus-like particles of a gammaretrovirus, the murine leukemia virus XMRV. J Virol 86:1297-306
Srb, Pavel; Vlach, Jiri; Prchal, Jan et al. (2011) Oligomerization of a retroviral matrix protein is facilitated by backbone flexibility on nanosecond time scale. J Phys Chem B 115:2634-44
Vorackova, Irena; Suchanova, Sarka; Ulbrich, Pavel et al. (2011) Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography. Protein Expr Purif 79:88-95
Prchal, Jan; Junkova, Petra; Strmiskova, Miroslava et al. (2011) Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements. Protein Expr Purif 79:122-7
Bohmova, Karolina; Hadravova, Romana; Stokrova, Jitka et al. (2010) Effect of dimerizing domains and basic residues on in vitro and in vivo assembly of Mason-Pfizer monkey virus and human immunodeficiency virus. J Virol 84:1977-88
Knejzlik, Zdenýýk; Ulbrich, Pavel; Strohalm, Martin et al. (2009) Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization. Virology 393:168-76

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