Mussel adhesive proteins (MAPs) are remarkable underwater adhesive polymers that form tenacious bonds to anchor marine organisms onto the substrates upon which they reside. Even in the presence of water, the adhesive protein plaques form extremely tenacious bonds to solid objects, an accomplishment which is not often matched by synthetic adhesives. Because of these properties as well as many similarities between the marine and physiologic environment, there is great interest in mimicking MAPs in synthetic polymers for use as adhesives in dentistry and medicine. However, these efforts are hampered by a lack of detailed understanding of the molecular mechanism of MAP adhesion. The goals of this research are to employ nano-, micro- and macro-scale adhesion experiments to gain a detailed understanding of the adhesive role of L-3,4-dihydroxyphenylalanine (DOPA) and other key residues in mussel adhesion, and to use this information to motivate the design of new MAP-inspired macromolecular biomaterials. Single molecule force probe measurements will be performed to investigate the bond forces and energies associated with interaction of DOPA and DOPA-containing peptides with representative implant materials as well as hard and soft tissues. To complement the single molecule experiments, the adhesive strength arising from contact of ensembles of peptides presented at the surfaces of hydrogels with implant materials and tissues will be tested using a fracture micromechanics methodology. Finally, the information obtained from the nano- and micro-scale adhesion experiments will be exploited for rational design of new MAP mimetic polymers. These polymers will be synthesized and tested as hard and soft tissue adhesives using macro-scale lap shear bond strength measurements. This study will provide new insights into the fundamental role of DOPA and other amino acids in biological adhesion, and will generate new adhesive biomaterials for use in soft and hard tissue repair, tissue regeneration, and drug delivery.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Method to Extend Research in Time (MERIT) Award (R37)
Project #
Application #
Study Section
Musculoskeletal Tissue Engineering Study Section (MTE)
Program Officer
Drummond, James
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Northwestern University at Chicago
Biomedical Engineering
Schools of Engineering
United States
Zip Code
Lee, Kyueui; Park, Eunsook; Lee, Haesung A et al. (2017) Phenolic condensation and facilitation of fluorescent carbon dot formation: a mechanism study. Nanoscale 9:16596-16601
Walsh, Pamela J; Clarke, Susan A; Julius, Matthew et al. (2017) Exploratory Testing of Diatom Silica to Map the Role of Material Attributes on Cell Fate. Sci Rep 7:14138
Gei├čler, Sebastian; Barrantes, Alejandro; Tengvall, Pentti et al. (2016) Deposition Kinetics of Bioinspired Phenolic Coatings on Titanium Surfaces. Langmuir 32:8050-60
Alves, Diana; Sileika, Tadas; Messersmith, Phillip B et al. (2016) Polydopamine-Mediated Immobilization of Alginate Lyase to Prevent P. aeruginosa Adhesion. Macromol Biosci 16:1301-10
Vidal, Cristina M P; Zhu, Weiying; Manohar, Suresh et al. (2016) Collagen-collagen interactions mediated by plant-derived proanthocyanidins: A spectroscopic and atomic force microscopy study. Acta Biomater 41:110-8
Zhou, Jiajing; Xiong, Qirong; Ma, Jielin et al. (2016) Polydopamine-Enabled Approach toward Tailored Plasmonic Nanogapped Nanoparticles: From Nanogap Engineering to Multifunctionality. ACS Nano 10:11066-11075
Li, Qiaochu; Barrett, Devin G; Messersmith, Phillip B et al. (2016) Controlling Hydrogel Mechanics via Bio-Inspired Polymer-Nanoparticle Bond Dynamics. ACS Nano 10:1317-24
Tang, Christina; Amin, Devang; Messersmith, Phillip B et al. (2015) Polymer directed self-assembly of pH-responsive antioxidant nanoparticles. Langmuir 31:3612-20
Zelasko-Leon, Daria C; Fuentes, Christina M; Messersmith, Phillip B (2015) MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods. PLoS One 10:e0128756
Go, Kevin; Kim, Yeong; Lee, Andy H et al. (2015) Design of Novel Mixer and Applicator for Two-Component Surgical Adhesives. J Med Device 9:0450011-450016

Showing the most recent 10 out of 61 publications