Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
4R37DK028607-16
Application #
2138204
Study Section
Special Emphasis Panel (NSS)
Project Start
1981-07-01
Project End
1999-06-30
Budget Start
1996-09-01
Budget End
1997-06-30
Support Year
16
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Biochemistry
Type
Other Domestic Higher Education
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Chen, Yung-Han; Maity, Amarendra N; Frey, Perry A et al. (2013) Mechanism-based inhibition reveals transitions between two conformational states in the action of lysine 5,6-aminomutase: a combination of electron paramagnetic resonance spectroscopy, electron nuclear double resonance spectroscopy, and density functional J Am Chem Soc 135:788-94
Chen, Yung-Han; Maity, Amarendra N; Pan, Yu-Chiang et al. (2011) Radical stabilization is crucial in the mechanism of action of lysine 5,6-aminomutase: role of tyrosine-263? as revealed by electron paramagnetic resonance spectroscopy. J Am Chem Soc 133:17152-5
Ruzicka, Frank J; Frey, Perry A (2010) Kinetic and spectroscopic evidence of negative cooperativity in the action of lysine 2,3-aminomutase. J Phys Chem B 114:16118-24
Maity, Amarendra N; Hsieh, Chih-Pin; Huang, Ming-Hui et al. (2009) Evidence for conformational movement and radical mechanism in the reaction of 4-thia-L-lysine with lysine 5,6-aminomutase. J Phys Chem B 113:12161-3
Tang, Kuo-Hsiang; Mansoorabadi, Steven O; Reed, George H et al. (2009) Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase. Biochemistry 48:8151-60
Schwartz, Phillip A; Lobrutto, Russell; Reed, George H et al. (2007) Probing interactions from solvent-exchangeable protons and monovalent cations with the 1,2-propanediol-1-yl radical intermediate in the reaction of dioldehydrase. Protein Sci 16:1157-64
Frey, Perry A; Hegeman, Adrian D; Reed, George H (2006) Free radical mechanisms in enzymology. Chem Rev 106:3302-16
Hinckley, Glen T; Frey, Perry A (2006) An adaptable spectroelectrochemical titrator: the midpoint reduction potential of the iron-sulfur center in lysine 2,3-aminomutase. Anal Biochem 349:103-11
Lees, Nicholas S; Chen, Dawei; Walsby, Charles J et al. (2006) How an enzyme tames reactive intermediates: positioning of the active-site components of lysine 2,3-aminomutase during enzymatic turnover as determined by ENDOR spectroscopy. J Am Chem Soc 128:10145-54
Lepore, Bryan W; Ruzicka, Frank J; Frey, Perry A et al. (2005) The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale. Proc Natl Acad Sci U S A 102:13819-24

Showing the most recent 10 out of 56 publications