of Research Plan: Our primary objective is to develop technetium (99mTc) and fluorine ('*F) renal tracers with a clearance in humans at least equal to that of the clinical gold standard, ^'l or//zo-iodohippuran ('^'l-OIH) and approaching the clearance of para-aminohippurate (PAH). Such agents should replace, the current best tracer, 99mTc Tcinercaptoacetyltriglycine (99mTc-MAGS). As detailed in this submission, we have made excellent progress in spite of the challenges posed by the disruption of the worldwide supply of iTiolybdenum-99 (the parent isotope of 99mTc) due to a temporary shutdown of aging reactors. To address a future shortage of 99mTc and to take advantage of the inherently better resolution of PET and the widespread availability of PET scanners, we are extending our original specific aims to use innovative chemistry and ligand design to develop renal tracers labeled with 18F as well as 99mTc. Our secondary objective, which will be achieved in the process of accomplishing our first objective, is to enhance the understanding of technetium and rhenium (Re) chemistry and to develop novel ligands using our extension of the one-step (Al18F)2+ method for 18F labeling of peptides in aqueous solution. The basic chemistry research in this project will facilitate the development by others of new non-renal integrated and bifunctional. 99mTc and 18F diagnostic radiopharmaceuticals as well as beta-emitting 186Re and 188Re e therapeutic radiopharmaceuticals. We hypothesize that ligands based on the relatively new {Tc1(C0)3} core and structural analogues of the Al18FNODA-butyric acid complex offer the best avenue for continued breakthrough research and enhanced diagnostic accuracy. During the first 4 years of funding, we propose to continue to test these hypotheses and derivative hypotheses by using an innovative and iterative combination of chemical methods, efficient routes to versatile novel ligands, and streamlined animal models. Simultaneously, throughout years 1-5, the direction of these studies and generation of new hypotheses will be guided by our animal results, by results comparing new tracers with 131I-OIH in both normal human subjects and those with reduced renal function, and by results comparing new tracers with 99mTc-MAG3 in subjects with impaired renal function and suspected obstruction.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Method to Extend Research in Time (MERIT) Award (R37)
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No Study Section (in-house review) (NSS)
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Moxey-Mims, Marva M
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Emory University
Schools of Medicine
United States
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Taylor, Andrew T; Folks, Russell D; Rahman, A K M Fazlur et al. (2017) 99mTc-MAG3: Image Wisely. Radiology 284:200-209
Lipowska, Malgorzata; Klenc, Jeffrey; Jarkas, Nashwa et al. (2017) Monoanionic 99mTc-tricarbonyl-aminopolycarboxylate complexes with uncharged pendant groups: Radiosynthesis and evaluation as potential renal tubular tracers. Nucl Med Biol 47:48-55
Klenc, Jeffrey; Lipowska, Malgorzata; Abhayawardhana, Pramuditha L et al. (2015) Structure and Properties of fac-[Re(I)(CO)3(NTA)](2-) (NTA(3-) = Trianion of Nitrilotriacetic Acid) and fac-[Re(I)(CO)3(L)](n-) Analogues Useful for Assessing the Excellent Renal Clearance of the fac-[(99m)Tc(I)(CO)3(NTA)](2-) Diagnostic Renal Agent. Inorg Chem 54:6281-90
Klenc, Jeffrey; Lipowska, Malgorzata; Taylor, Andrew T (2015) Identification of lead compounds for (99m)Tc and (18)F GPR91 radiotracers. Bioorg Med Chem Lett 25:2335-9
Lipowska, Malgorzata; Klenc, Jeffrey; Shetty, Dinesh et al. (2014) Al18F-NODA-butyric acid: biological evaluation of a new PET renal radiotracer. Nucl Med Biol 41:248-53
Abhayawardhana, Pramuditha L; Marzilli, Patricia A; Fronczek, Frank R et al. (2014) Complexes possessing rare ""tertiary"" sulfonamide nitrogen-to-metal bonds of normal length: fac-[Re(CO)3(N(SO2R)dien)]PF6 complexes with hydrophilic sulfonamide ligands. Inorg Chem 53:1144-55
Taylor, Andrew T (2014) Radionuclides in nephrourology, Part 2: pitfalls and diagnostic applications. J Nucl Med 55:786-98
Lipowska, Malgorzata; Klenc, Jeffrey; Folks, Russell D et al. (2014) Initial Evaluation of (99m)Tc(CO)3(ASMA) as a Renal Tracer in Healthy Human Volunteers. Nucl Med Mol Imaging 48:216-24
Taylor, Andrew T (2014) Radionuclides in nephrourology, part 1: Radiopharmaceuticals, quality control, and quantitative indices. J Nucl Med 55:608-15
Taylor, Andrew T; Lipowska, Malgorzata; Cai, Hui (2013) 99mTc(CO)3(NTA) and 131I-OIH: comparable plasma clearances in patients with chronic kidney disease. J Nucl Med 54:578-84

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