We have made substantial progress on several of the Specific Aims proposed in 2006 and further focused future directions toward the challenging long-time aim of understanding eukaryotic channel gating by ligands, particularly in the Acetylcholine Receptor (AcChR). A major subtext has always been to attempt to define structures of eukaryotic and human proteins because of their importance for drug discovery. Towards this goal, we determined two of the first four structures for human membrane proteins, using expression in human cultured HEK293S cells.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Method to Extend Research in Time (MERIT) Award (R37)
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Special Emphasis Panel (NSS)
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Chin, Jean
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University of California San Francisco
Schools of Medicine
San Francisco
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Kapoor, Khyati; Finer-Moore, Janet S; Pedersen, Bjørn P et al. (2016) Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides. Proc Natl Acad Sci U S A 113:4711-6
Thurtle-Schmidt, Bryan H; Stroud, Robert M (2016) Structure of Bor1 supports an elevator transport mechanism for SLC4 anion exchangers. Proc Natl Acad Sci U S A 113:10542-6
Kintzer, Alexander F; Stroud, Robert M (2016) Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature 531:258-62
Salo-Ahen, Outi M H; Tochowicz, Anna; Pozzi, Cecilia et al. (2015) Hotspots in an obligate homodimeric anticancer target. Structural and functional effects of interfacial mutations in human thymidylate synthase. J Med Chem 58:3572-81
Tochowicz, Anna; Santucci, Matteo; Saxena, Puneet et al. (2015) Alanine mutants of the interface residues of human thymidylate synthase decode key features of the binding mode of allosteric anticancer peptides. J Med Chem 58:1012-8
Johri, Atul K; Oelmüller, Ralf; Dua, Meenakshi et al. (2015) Fungal association and utilization of phosphate by plants: success, limitations, and future prospects. Front Microbiol 6:984
Kim, JungMin; Wu, Shenping; Tomasiak, Thomas M et al. (2015) Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature 517:396-400
Rosenberg, Oren S; Dovala, Dustin; Li, Xueming et al. (2015) Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion. Cell 161:501-512
Kumar, Hemant; Finer-Moore, Janet S; Kaback, H Ronald et al. (2015) Structure of LacY with an ?-substituted galactoside: Connecting the binding site to the protonation site. Proc Natl Acad Sci U S A 112:9004-9
Monk, Brian C; Tomasiak, Thomas M; Keniya, Mikhail V et al. (2014) Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer. Proc Natl Acad Sci U S A 111:3865-70

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