Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM024689-19
Application #
2174308
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1978-01-01
Project End
1998-12-31
Budget Start
1996-01-01
Budget End
1996-12-31
Support Year
19
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Biochemistry
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Meier, Katlyn K; Rogers, Melanie S; Kovaleva, Elena G et al. (2016) Enzyme Substrate Complex of the H200C Variant of Homoprotocatechuate 2,3-Dioxygenase: Mössbauer and Computational Studies. Inorg Chem 55:5862-70
Meier, Katlyn K; Rogers, Melanie S; Kovaleva, Elena G et al. (2015) A Long-Lived Fe(III)-(Hydroperoxo) Intermediate in the Active H200C Variant of Homoprotocatechuate 2,3-Dioxygenase: Characterization by Mössbauer, Electron Paramagnetic Resonance, and Density Functional Theory Methods. Inorg Chem 54:10269-80
Kovaleva, Elena G; Rogers, Melanie S; Lipscomb, John D (2015) Structural Basis for Substrate and Oxygen Activation in Homoprotocatechuate 2,3-Dioxygenase: Roles of Conserved Active Site Histidine 200. Biochemistry 54:5329-39
Knoot, Cory J; Purpero, Vincent M; Lipscomb, John D (2015) Crystal structures of alkylperoxo and anhydride intermediates in an intradiol ring-cleaving dioxygenase. Proc Natl Acad Sci U S A 112:388-93
Rivard, Brent S; Rogers, Melanie S; Marell, Daniel J et al. (2015) Rate-Determining Attack on Substrate Precedes Rieske Cluster Oxidation during Cis-Dihydroxylation by Benzoate Dioxygenase. Biochemistry 54:4652-64
Fielding, Andrew J; Lipscomb, John D; Que Jr, Lawrence (2014) A two-electron-shell game: intermediates of the extradiol-cleaving catechol dioxygenases. J Biol Inorg Chem 19:491-504
Lipscomb, John D (2014) Life in a sea of oxygen. J Biol Chem 289:15141-53
Yin, DeLu Tyler; Purpero, Vince M; Fujii, Ryota et al. (2013) New structural motif for carboxylic acid perhydrolases. Chemistry 19:3037-46
Hayden, Joshua A; Farquhar, Erik R; Que, Lawrence et al. (2013) NO binding to Mn-substituted homoprotocatechuate 2,3-dioxygenase: relationship to Oýýý reactivity. J Biol Inorg Chem 18:717-28
Kovaleva, Elena G; Lipscomb, John D (2012) Structural basis for the role of tyrosine 257 of homoprotocatechuate 2,3-dioxygenase in substrate and oxygen activation. Biochemistry 51:8755-63

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