The long-term goal of our work is to understand the molecular mechanisms that control eukaryotic mRNA stability, which is a critical step in gene regulation. One major pathway of mRNA turnover in eukaryotes initiates with poly(A) tail shortening, which triggers decapping, leading to 5'to 3'exonucleolysis. Decapping is a key step in this pathway because it induces degradation of the mRNA, and is the site of numerous control inputs including the poly(A) tail and specific sequences that modulate mRNA decapping rate. Given this importance, we focused on understanding the mechanisms of mRNA decapping. In the past funding period, we clarified the nature of the decapping enzyme, identified the mechanisms by which some proteins affect the rate of decapping, and provided strong evidence that a key step in decapping is exiting the translating pool of mRNAs coupled with a specific mRNP re-arrangement. In addition, we also identified novel cytoplasmic structures (referred to as P-bodies) where the mRNA decapping machinery is concentrated and where mRNA degradation can occur. P-bodies are significant because: 1) they imply unanticipated compartmentalization of mRNA decay, which becomes a possible point of mRNA regulation, 2) are conserved in other eukaryotes and 3) are likely to be sites of additional events in mRNA biology, possibly including storage of translationally repressed mRNAs. In this grant, we will continue our analysis of mRNA decapping with a focus on understanding the composition, properties, and function of P-bodies.
The specific aims are as follows: I) Determine the composition and ultrastructure of P-bodies II) Analyze P-body assembly III) Analyze the function of the Dhhl and Pat1 proteins IV) Determine if P-bodies function in other aspects of cytoplasmic mRNA physiology.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM045443-24
Application #
8440777
Study Section
Special Emphasis Panel (NSS)
Program Officer
Bender, Michael T
Project Start
1991-04-01
Project End
2014-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
24
Fiscal Year
2013
Total Cost
$304,923
Indirect Cost
$101,576
Name
University of Colorado at Boulder
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309
Jain, Saumya; Wheeler, Joshua R; Walters, Robert W et al. (2016) ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 164:487-98
Mitchell, Sarah F; Parker, Roy (2016) Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment. Methods Mol Biol 1421:153-63
Lin, Yuan; Protter, David S W; Rosen, Michael K et al. (2015) Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins. Mol Cell 60:208-19
Mitchell, Sarah F; Parker, Roy (2014) Principles and properties of eukaryotic mRNPs. Mol Cell 54:547-58
Lasda, Erika; Parker, Roy (2014) Circular RNAs: diversity of form and function. RNA 20:1829-42
Walters, Robert W; Shumilin, Igor A; Yoon, Je-Hyun et al. (2014) Edc3 function in yeast and mammals is modulated by interaction with NAD-related compounds. G3 (Bethesda) 4:613-22
Shukla, Siddharth; Parker, Roy (2014) Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion. Proc Natl Acad Sci U S A 111:E3277-86
Ramaswami, Mani; Taylor, J Paul; Parker, Roy (2013) Altered ribostasis: RNA-protein granules in degenerative disorders. Cell 154:727-36
Mitchell, Sarah F; Jain, Saumya; She, Meipei et al. (2013) Global analysis of yeast mRNPs. Nat Struct Mol Biol 20:127-33
Jain, Saumya; Parker, Roy (2013) The discovery and analysis of P Bodies. Adv Exp Med Biol 768:23-43

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