The long-term goal of our research is to dissect the cellular and biochemical basis of this light control of development switch. Our previous work, together with studies from others, has defined a group of evolutionary conserved COP/DET/FUS proteins forming three major protein complexes that act as light nactivatable represser of photomorphogenesis. We have established that those three complexes act within nucleus to suppress the photomorphogenic developmental pathway in darkness and light reverses this repressive action, in part by depleting COP1 nuclear accumulation. In the nucleus, COP1 complexes act as E3 ligase, physically contact and target specific transcription factors (including HY5 and HYH) for ubiquitination and proteasome mediated degradation. The activities of those transcription factors are responsible for promoting photomorphogenic development and gene expression.While CDD complex (COP10, DET1, and DDB1) works as a component of a novel CUL4-Rbx1 based E3 ligase whose activity depends on functional COP9 signalosome and positively regulates the COP1 complexes. We further established that phosphorylation events in phytochrome A and its downstream partner FHY1 play critical roles in far red light control of development and their functional interactions with COP1 complexes. Here I propose to continue our combinatorial approaches to comprehensively analyze how this regulatory network mediates light control of genome expression and development. To achieve this goal, I proposed three major complementary aspects of further research with nine specific aims. The first aspect, including Aims 1-3, is to reveal the biochemical activities underlining the functional interaction among the E3 ligase complexes and the COP9 signalosome. The second aspect, including Aims 4-6, is to understand the biochemical and molecular events defining functional interplay between the COP1 E3 ligase complexes and phytochrome A signaling pathway. The third aspect, including Aims 7-9, is to provide a whole genome level understanding on light control of gene expression and reveal the transcriptional cascade/network and chromatin remodeling events at a genomic scale. The accomplishment of these proposed objectives shall provide a comprehensive and mechanistic view of the signaling network responsible for light control of development. This should serve as an essential guidance for investigating similar biochemical entities in mammals including human.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM047850-21
Application #
8214549
Study Section
Special Emphasis Panel (NSS)
Program Officer
Maas, Stefan
Project Start
1992-08-01
Project End
2014-01-31
Budget Start
2012-02-01
Budget End
2013-01-31
Support Year
21
Fiscal Year
2012
Total Cost
$585,069
Indirect Cost
$228,763
Name
Yale University
Department
Physiology
Type
Schools of Arts and Sciences
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Shi, Hui; Shen, Xing; Liu, Renlu et al. (2016) The Red Light Receptor Phytochrome B Directly Enhances Substrate-E3 Ligase Interactions to Attenuate Ethylene Responses. Dev Cell 39:597-610
Shi, Hui; Liu, Renlu; Xue, Chang et al. (2016) Seedlings Transduce the Depth and Mechanical Pressure of Covering Soil Using COP1 and Ethylene to Regulate EBF1/EBF2 for Soil Emergence. Curr Biol 26:139-49
Xu, Dongqing; Jiang, Yan; Li, Jigang et al. (2016) BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation. Proc Natl Acad Sci U S A 113:7655-60
Li, Kunlun; Yu, Renbo; Fan, Liu-Min et al. (2016) DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis. Nat Commun 7:11868
Sun, Ning; Wang, Jiajun; Gao, Zhaoxu et al. (2016) Arabidopsis SAURs are critical for differential light regulation of the development of various organs. Proc Natl Acad Sci U S A 113:6071-6
Zhu, Ling; Bu, Qingyun; Xu, Xiaosa et al. (2015) CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1. Nat Commun 6:7245
Huang, Hao; Yang, Mei; Su, Yan'e et al. (2015) Arabidopsis Atypical Kinases ABC1K1 and ABC1K3 Act Oppositely to Cope with Photodamage Under Red Light. Mol Plant 8:1122-4
Kim, Sang-Hoon; Kim, Hani; Seo, Kyoung-In et al. (2014) DWD HYPERSENSITIVE TO UV-B 1 is negatively involved in UV-B mediated cellular responses in Arabidopsis. Plant Mol Biol 86:571-83
Zhong, Shangwei; Shi, Hui; Xue, Chang et al. (2014) Ethylene-orchestrated circuitry coordinates a seedling's response to soil cover and etiolated growth. Proc Natl Acad Sci U S A 111:3913-20
Xu, Xiaosa; Paik, Inyup; Zhu, Ling et al. (2014) PHYTOCHROME INTERACTING FACTOR1 Enhances the E3 Ligase Activity of CONSTITUTIVE PHOTOMORPHOGENIC1 to Synergistically Repress Photomorphogenesis in Arabidopsis. Plant Cell 26:1992-2006

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