The long-term goal of our research is to dissect the cellular and biochemical basis of this light control of development switch. Our previous work, together with studies from others, has defined a group of evolutionary conserved COP/DET/FUS proteins forming three major protein complexes that act as light nactivatable represser of photomorphogenesis. We have established that those three complexes act within nucleus to suppress the photomorphogenic developmental pathway in darkness and light reverses this repressive action, in part by depleting COP1 nuclear accumulation. In the nucleus, COP1 complexes act as E3 ligase, physically contact and target specific transcription factors (including HY5 and HYH) for ubiquitination and proteasome mediated degradation. The activities of those transcription factors are responsible for promoting photomorphogenic development and gene expression.While CDD complex (COP10, DET1, and DDB1) works as a component of a novel CUL4-Rbx1 based E3 ligase whose activity depends on functional COP9 signalosome and positively regulates the COP1 complexes. We further established that phosphorylation events in phytochrome A and its downstream partner FHY1 play critical roles in far red light control of development and their functional interactions with COP1 complexes. Here I propose to continue our combinatorial approaches to comprehensively analyze how this regulatory network mediates light control of genome expression and development. To achieve this goal, I proposed three major complementary aspects of further research with nine specific aims. The first aspect, including Aims 1-3, is to reveal the biochemical activities underlining the functional interaction among the E3 ligase complexes and the COP9 signalosome. The second aspect, including Aims 4-6, is to understand the biochemical and molecular events defining functional interplay between the COP1 E3 ligase complexes and phytochrome A signaling pathway. The third aspect, including Aims 7-9, is to provide a whole genome level understanding on light control of gene expression and reveal the transcriptional cascade/network and chromatin remodeling events at a genomic scale. The accomplishment of these proposed objectives shall provide a comprehensive and mechanistic view of the signaling network responsible for light control of development. This should serve as an essential guidance for investigating similar biochemical entities in mammals including human.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM047850-21
Application #
8214549
Study Section
Special Emphasis Panel (NSS)
Program Officer
Maas, Stefan
Project Start
1992-08-01
Project End
2014-01-31
Budget Start
2012-02-01
Budget End
2013-01-31
Support Year
21
Fiscal Year
2012
Total Cost
$585,069
Indirect Cost
$228,763
Name
Yale University
Department
Physiology
Type
Schools of Arts and Sciences
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Chen, Fang; Li, Bosheng; Demone, Jordan et al. (2014) Photoreceptor partner FHY1 has an independent role in gene modulation and plant development under far-red light. Proc Natl Acad Sci U S A 111:11888-93
Ouyang, Xinhao; Huang, Xi; Jin, Xiao et al. (2014) Coordinated photomorphogenic UV-B signaling network captured by mathematical modeling. Proc Natl Acad Sci U S A 111:11539-44
Lau, On Sun; Huang, Xi; Charron, Jean-Benoit et al. (2011) Interaction of Arabidopsis DET1 with CCA1 and LHY in mediating transcriptional repression in the plant circadian clock. Mol Cell 43:703-12
Zhou, Junli; Wang, Xiangfeng; He, Kun et al. (2010) Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression. Plant Mol Biol 72:585-95
Bai, Sulan; Zhao, Jinyi; Zhang, Yaochuan et al. (2010) Rapid and reliable detection of 11 food-borne pathogens using thin-film biosensor chips. Appl Microbiol Biotechnol 86:983-90
Lau, On Sun; Deng, Xing Wang (2010) Plant hormone signaling lightens up: integrators of light and hormones. Curr Opin Plant Biol 13:571-7
Feng, Suhua; Martinez, Cristina; Gusmaroli, Giuliana et al. (2008) Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature 451:475-9
Yu, Jae-Woong; Rubio, Vicente; Lee, Na-Yeoun et al. (2008) COP1 and ELF3 control circadian function and photoperiodic flowering by regulating GI stability. Mol Cell 32:617-30
Saijo, Yusuke; Zhu, Danmeng; Li, Jigang et al. (2008) Arabidopsis COP1/SPA1 complex and FHY1/FHY3 associate with distinct phosphorylated forms of phytochrome A in balancing light signaling. Mol Cell 31:607-13
Menon, Suchithra; Chi, Hongbo; Zhang, Huiyong et al. (2007) COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence. Nat Immunol 8:1236-45

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