Mass spectrometry-based proteomics is an emerging technology that has seen possible applications everywhere. The technique can potentially use one drop of our blood to reveal onset and progress of diseases. Current R&D using plasma/serum proteome sample sets, however, is severely limited by the high complexity and dynamic range of protein concentrations. Vast majority of plasma/serum proteomic strategies lack adequate reproducibility, sensitivity, and robustness for clinical tests. Solutions for these serious technological barriers to develop a reliable detection platform toward clinical implementation are elusive. In this NIH STTR Phase I study, we will develop a novel analytical platform based on functionalized soluble nanopolymers into commercial products for simple, routine serum/plasma biomarker discovery. Through this STTR Phase I project, we will leap from our expertise in pioneering the application of soluble nanopolymers to establish Polymer-based Metal Ion Affinity Capture (PolyMAC) functionalized with different metal ions as a powerful platform for sequential depletion of high-abundant proteins and isolation of low abundant plasma proteins to achieve sensitive, reproducible and quantitative plasma proteomics. In particular, we will validate the platform through analyzing plasma samples including those from Ossabaw swine, a distinctive large animal model for early detection of coronary artery disease (CAD). In order to achieve these goals, the following aims will be completed:
Aim #1 : Optimization of PolyMAC-Cu for high-abundant plasma protein depletion.
Aim #2 : Optimization of in-depth plasma proteome analyses using sequential PolyMAC-M isolations. By the completion of STTR Phase I studies, we expect to develop a versatile sample preparation platform with core reagents and kits for plasma proteomics and to facilitate translating proteomics discovery to clinical applications.

Public Health Relevance

Discovery of clinically useful biomarkers can offer enormous potential for modern medicine by providing earlier and better disease diagnosis, improved prognosis for patient monitoring, and preventative screening that can identify patients at highest risk. This NIH STTR will support an effort to develop an innovative technology into commercial products that equip researchers with powerful tools and new directions in biomarker discovery field.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Technology Transfer (STTR) Grants - Phase I (R41)
Project #
1R41GM109626-01A1
Application #
8780252
Study Section
Special Emphasis Panel (ZRG1-IMST-M (13))
Program Officer
Anderson, Vernon
Project Start
2014-09-25
Project End
2016-08-31
Budget Start
2014-09-25
Budget End
2015-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$312,537
Indirect Cost
Name
Tymora Analytical Operations, LLC
Department
Type
DUNS #
965433258
City
West Lafayette
State
IN
Country
United States
Zip Code
47906