Abstract

This technology is intended to reduce the costs of sequencing by improving the ability to sequence through difficult templates such as G/C or A/T rich regions, direct and inverted repeats, homopolymer tracts, and di, and tri-nucleotide repeats. This will decrease finishing costs by reducing gaps. Additional improvements will enable direct sequencing of colony derived plasmids and more robust sequencing of BACs, cosmids and genomic DNA. These objectives will be attained by examining an entirely new class of DNA polymerases as improved sequencing reagents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43HG002714-01
Application #
6583877
Study Section
Special Emphasis Panel (ZRG1-SSS-Y (10))
Program Officer
Graham, Bettie
Project Start
2002-09-27
Project End
2004-03-26
Budget Start
2002-09-27
Budget End
2004-03-26
Support Year
1
Fiscal Year
2002
Total Cost
$99,510
Indirect Cost

  Institution

Name
Lucigen Corporation
Department
Type
DUNS #
019710669
City
Middleton
State
WI
Country
United States
Zip Code
53562

  Publications

Schoenfeld, Thomas W; Murugapiran, Senthil K; Dodsworth, Jeremy A et al. (2013) Lateral gene transfer of family A DNA polymerases between thermophilic viruses, aquificae, and apicomplexa. Mol Biol Evol 30:1653-64
Moser, Michael J; DiFrancesco, Robert A; Gowda, Krishne et al. (2012) Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme. PLoS One 7:e38371
Pride, David T; Schoenfeld, Thomas (2008) Genome signature analysis of thermal virus metagenomes reveals Archaea and thermophilic signatures. BMC Genomics 9:420
Schoenfeld, Thomas; Patterson, Melodee; Richardson, Paul M et al. (2008) Assembly of viral metagenomes from yellowstone hot springs. Appl Environ Microbiol 74:4164-74