MALDI tissue imaging, when enabled by Nanoparticulate (NP) Matrix implantation, is maturing into a useful preclinical tool for biomarker and drug discovery in our laboratory and at NIDA-IRP. Quantitatively reproducible images from serial coronal sections suggest that relative quantitation is now possible in rodent models of disease and injury. Nanoparticulate (NP) matrix implantation allows numerical quantitation of differences in biomarker levels in rodent disease and injury models. No commercial instrument which performs mass spectral molecular imaging of tissues has software capable of analyzing and displaying these quality data sets. 1) Software and hardware developed under our antecedent phase II for analyzing the multidimensional data from our MALDI-Ion Mobility-MS imaging system will be expanded to serve this need and will also have dual use in correlating molecular image quality with the nano-scale characterization of the NP matrices by advance materials analysis techniques such as the subnanometer resolution Zeiss Orion Helium Ion Microscope. 2) The continued growth of unique core shell NP matrices will continue to be aided by advanced materials analysis measurement of the NP structures and particle size distributions after implantation into the tissue. 3) Protocols for standards incorporation into and onto the tissue surface will attempt to prove the capability of relative and absolute quantitation in MALDI-MS imaging.

Public Health Relevance

Nanoparticulate (NP) matrix implantation numerical quantitation of differences in biomarker levels in rodent disease and injury models may be possible. No commercial instrument which performs imaging has software capable of analyzing and displaying these data sets. Software developed under a previous phase II for our Ion mobility system can be modified to fulfill these needs for other instruments. Growth of new NPs, advanced characterization, and biological imaging will occur in parallel.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44DA036263-03
Application #
8661740
Study Section
Special Emphasis Panel (ZRG1-ETTN-A (10))
Program Officer
Singh, Hari
Project Start
2013-06-01
Project End
2016-05-31
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
3
Fiscal Year
2014
Total Cost
$998,797
Indirect Cost
Name
Ionwerks, Inc.
Department
Type
DUNS #
154074553
City
Houston
State
TX
Country
United States
Zip Code
77002