Universal newborn hearing screening is mandated in the US and is performed in the postpartum hospital setting. Current physiology-based hearing screening tests are unable to detect late-onset hearing loss caused by cytomegalovirus (CMV) infection. Therefore, a complementary screening approach has been suggested to prevent disabilities associated with CMV-related hearing loss by early detection and intervention. A nucleic acid test has been described to screen newborns for congenital CMV infection using saliva specimens where it is more sensitive than the dried blood spots traditionally used by the newborn screening laboratories. Newborn screening programs do not have the infrastructure necessary to use newborn saliva samples and molecular assays. Advanced Liquid Logic's digital microfluidic technology is a recognized innovative, low-cost, and automated liquid handling system that can address this challenge. The objective of this Fast-Track application is to develop an integrated lab-on-a-chip to perform screening of congenital CMV infection in newborns using saliva samples.
Specific aims i n Phase I are to develop protocols for CMV purification from saliva samples and for viral DNA amplification on-cartridge, and to integrate a "sample-to-answer" CMV screening assay on the digital microfluidic platform. Success in Phase I will result in a Phase II continuation where the specific aims will be (i) to develop a point-of-care newborn screening platform, (ii) to optimize the "sample-to-answer" CMV protocol in this platform and assess its analytical performance, and (iii) to perform a method comparison with the "gold standard" rapid saliva culture using neonatal saliva samples in collaboration with the University of Alabama at Birmingham.

Public Health Relevance

Newborn hearing screening is performed on almost every baby born in the US but some infants develop hearing loss caused by cytomegalovirus (CMV) infection after the baby passes the current hearing screening test. Therefore, a complementary screening approach has been suggested to prevent CMV-related hearing loss by early detection and intervention. In this project, a digital microfluidic platform will be developed to perform CMV screening at the point-of-care using a saliva sample. Such technology can significantly impact the health outcomes for infants with increased risk of hearing loss caused by congenital CMV infection.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
4R44DC012967-02
Application #
8653807
Study Section
Special Emphasis Panel (ZRG1-ETTN-G (12))
Program Officer
Miller, Roger
Project Start
2012-12-01
Project End
2015-05-31
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
2
Fiscal Year
2013
Total Cost
$681,273
Indirect Cost
Name
Advanced Liquid Logic
Department
Type
DUNS #
140695474
City
Research Triangle Park
State
NC
Country
United States
Zip Code
27709