The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of blood typing assays. The conventional hemagglutination blood typing assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propose to develop a new technology, which is free from the aforementioned limitations of the conventional hemagglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation.
The Specific Aims of Phase II are: 1) To further explore all variables affecting the performance of the new blood typing detection assays and optimize the performance of the assay; 2) To design and assemble an ?rototype of the device for performing of several blood typing assays simultaneously. The ?rototype is intended to test the design of every technical element of the system before it is implemented in the final pre- production prototype; 3) To validate the detection assays for determination of blood groups (including weak D group and C, c, E, e and K antigens) and expected antibodies (reverse typing); To examine a statistically significant number of clinical samples for each assay, and carry out statistical analysis and demonstrate reproducibility of the obtained results; To compare performance of the conventional and newly developed assays. Significantly, the new method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silic microparticles, and biological cells.

Public Health Relevance

The development of this method will allow one to obtain quantitative results, increase the sensitivity of red blood cell agglutination assays, and reduce time of analysis without compromising the cost per test. Successful completion of these studies will result in a substantial business opportunity to license the technology and manufacture commercial products, which will be beneficial for public health.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44HL117557-03
Application #
9127323
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Mitchell, Phyllis
Project Start
2013-02-01
Project End
2017-04-30
Budget Start
2016-05-01
Budget End
2017-04-30
Support Year
3
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Allied Innovative Systems, LLC
Department
Type
DUNS #
139604156
City
Hillsborough
State
NJ
Country
United States
Zip Code