Chlamydia trachomatis is an intracellular bacterium that is a major cause of sexually transmitted diseases and infectious blindness. Chlamydia's survival largely relies on two fundamental processes 1) protecting its infectious intracellular compartment (so-called inclusion) from the lysosomal degradative pathway and 2) hijacking host compartments to acquire nutrients and material necessary to grow the inclusion. To perform both these events, C. trachomatis evolved to be extraordinarily efficient at manipulating membrane fusion and promote its survival. Despite the importance of this intimate connection between Chlamydia and host vesicular trafficking, very little is known regarding the impact of this bacterium on the host membrane fusion machinery, the so-called SNAREs. Do Chlamydiae extensively co-opt SNAREs to control the host vesicular trafficking and promote their survival inside the cell? Using an innovative multidisciplinary approach, we seek to determine whether C. trachomatis (i) uses multiple SNARE-like proteins to sequentially block different host SNARE complexes involved in the endocytic/lysosomal pathway and protect its inclusion from destruction and (ii) recruits host SNAREs involved in the secretory pathway to hijack host vesicles and promote the growth of its inclusion. The characterization of multiple chlamydial SNARE-like proteins will give scientists the fundamental tools to screen for their presence in the proteome of other major human pathogens, including Salmonella typhi, and Mycobacterium tuberculosis, thus opening new avenues of research.

Public Health Relevance

Chlamydia trachomatis infections remain one of the most prevalent bacterial sexually transmitted diseases, and despite the availability of antibiotics, the number of infections has been increasing since the 1980s. Understanding how Chlamydia establishes a successful intracellular lifestyle after it invades host cells will facilitate the development of novel drugs that specifically target its pathogenic mechanisms. In particular, determining how Chlamydia co-opts host vesicular trafficking will provide powerful therapeutic opportunities.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
High Priority, Short Term Project Award (R56)
Project #
Application #
Study Section
Host Interactions with Bacterial Pathogens Study Section (HIBP)
Program Officer
Hiltke, Thomas J
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Thomas Jefferson University
Schools of Medicine
United States
Zip Code
Wesolowski, Jordan; Weber, Mary M; Nawrotek, Agata et al. (2017) Chlamydia Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning. MBio 8:
Weber, Mary M; Noriea, Nicholas F; Bauler, Laura D et al. (2016) A Functional Core of IncA Is Required for Chlamydia trachomatis Inclusion Fusion. J Bacteriol 198:1347-55
Bonne-Année, Sandra; Kerepesi, Laura A; Hess, Jessica A et al. (2014) Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis. Microbes Infect 16:502-11
Wesolowski, Jordan; Paumet, Fabienne (2014) Escherichia coli exposure inhibits exocytic SNARE-mediated membrane fusion in mast cells. Traffic 15:516-30