Mutations on cardiac ion channel genes can lead to electrical rhythm disturbances known as arrhythmias. Such inherited rhythm disturbances are termed ion channelopathies and include disorders such as Brugada Syndrome (BrS) and Long QT Syndrome (LQTS). In certain instances approximately 70% of channelopathy genes are elude detection. 'MAGUK'proteins scaffold ion channels underneath cell membranes, allowing for efficient electrical signaling. Abnormal expression of a MAGUK could alter ion channel function and thereby compromise electrical excitation. The prototypical MAGUK is the synapse-associated protein 97 (SAP97). SAP97 gene is abundantly expressed in the heart, and there is evidence that SAP97 interacts with ion channels and may therefore regulate excitation. We generated a unique mouse model with the gene knock out (Sap97-KO), to investigate the role of Sap97 in excitation. Sap97-KO mice have abnormalities in ECG and cell electrophysiological properties. Furthermore, a recent clinical study identified mutations in SAP97, which were associated with BrS, and probably the result of abnormally increased expression of a key potassium channel. It is our hypothesis that SAP97 is important for the assembly of cardiac ion channel proteins, and that abnormal SAP97 expression will increase arrhythmia susceptibility. We will test our hypothesis using our mouse model, as well as patient-specific stem cell-derived cardiac myocytes (iPSC-CMs).
Three aims will be addressed. 1) To study arrhythmogenic mechanisms in the myocardium of Sap97-KO mice. 2) To investigate Sap97- dependent changes in the expression of potassium, sodium and calcium channels in Sap97-KO mouse cells, and in stem cell-derived cardiac myocytes (iPSC-CMs) of patients with SAP97 mutations in BrS. 3) To study arrhythmia mechanisms associated with human SAP97 mutations in single cells, as well as in layers of iPSC- CMs. Our proposed study will provide insight into molecular mechanisms of channelopathies associated with abnormal SAP97 expression, as well as provide targets for therapeutic intervention.

Public Health Relevance

Ion channels are protein molecules responsible for electrical excitation. Mutations in channel genes can alter channel function and cause irregular heartbeats (arrhythmias), and may lead to fatality. 'MAGUK'proteins anchor channels in cell membranes and thus regulate channel function. Mutations in MAGUKs therefore may also cause arrhythmias. This proposal tests this hypothesis using our unique mouse model with abnormal expression of the major MAGUK protein (SAP97), as well as stem cell-derived heart cells from patients harboring SAP97 mutations.

Agency
National Institute of Health (NIH)
Type
High Priority, Short Term Project Award (R56)
Project #
1R56HL124319-01
Application #
8903572
Study Section
Electrical Signaling, Ion Transport, and Arrhythmias Study Section (ESTA)
Program Officer
Krull, Holly
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109