Funds for an Applied Biosystems / Life Technology 3500xL (24 capillary) DNA Analyzer are requested. It will become the principle instrument for the DNA sequencing and fragment analysis provided by the DNA Services Core Facility (://www.ohsu.edu/xd/research/research-cores/dna- analysis/). This well-established core facility provides capillary electrophoresis sequencing services to ~100 principle investigators in 23 OHSU departments, center and institutes, and 12 other non-OHSU Portland area departments. Long-read Sanger sequencing is a basic technique required by all labs performing modern, hypothesis-driven molecular biology. Massively parallel whole genome research is expected to increase the need for follow-up Sanger sequencing in the near term. We will run ~30,000 samples this year. With the requested instrument, we will be able to double that productivity. A few, of the more than 200 basic and translational NIH funded projects we support, are "Accelerating target identification and validation in leukemia" in the Brian Druker lab in the Knight Cancer Institute;"Treatment of Distal Tyrosine pathway Disorders" in the Markus Grompe lab in the Oregon Stem Cell Center;and "Genetic and molecular dissection of hair-cell function" in the Teresa Nicolson lab in the School of Medicine, Otolaryngology Research Department. Sequencing by our core for projects in Molecular Microbiology &Immunology, Infectious Disease, Ophthalmology, Cell Biology &Development and Nephrology departments in the School of Medicine, the Center for Research on Occupational &Environmental Toxicology (CROET) and the Biology Department at Portland State University round out our major user group;accounting for >55% of our current usage. The PI for this grant designed and implemented the databases and website used by the core for receiving work-requests, tracking work and performing billing and reporting functions. The technical staff, besides Dr. Keller, consists of one full-time technician with several years of automated DNA sequencing experience and one-half time technician. The current, 16 capillary 3130xL sequencer will be used for shorter, faster PCR sequencing runs;and for backup during maintenance and repair downtime. The requested 24 capillary instrument will be used for samples requiring the long reads (800-1000 bases) and fragment analysis. The requested instrumentation will significantly increase our data throughput and genomic analysis productivity.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD010609-01
Application #
8246286
Study Section
Special Emphasis Panel (ZRG1-GGG-H (30))
Program Officer
Levy, Abraham
Project Start
2012-05-04
Project End
2013-05-03
Budget Start
2012-05-04
Budget End
2013-05-03
Support Year
1
Fiscal Year
2012
Total Cost
$178,610
Indirect Cost
Name
Oregon Health and Science University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239