Funds are requested to purchase an Olympus Fluoview 1000S confocal instrument with state-of-the art functionality to replace an aging shared system that can no longer meet the research demands of our NIH funded researchers. There is a general demand for the increased speed and sensitivity that the Fluoview 1000S will provide. Additionally, collaborations across fields have broadened the research demands upon our facility and its shared confocal system. Our confocal system needs to support analyses of multiple organisms (e.g. marine invertebrates, C. elegans, and mammalian cell cultures), multiple probes (fluorescent proteins, quantum dots, and dyes) in multiple research paradigms. To provide this versatility, we have proposed a system with spectral imaging capabilities, a motorized X, Y stage and an environmental system that supports a range of imaging demands. This configuration will allow researchers to systematically sample large fields at high resolution, and to repeatedly sample multiple locations in fixed and live specimens. A replacement system is critical to insuring the on-going success of our NIH funded researchers and to supporting their biomedically relevant research programs.

Public Health Relevance

A number of NIH funded research projects depend on access to a state-of-the art confocal microscope. The replacement of an aging discontinued confocal microscope with the proposed state-of-the-art system will facilitate numerous biomedical research projects relevant to public health including projects on cancer, wound healing, polycystic kidney disease, diabetes and Alzheimer's disease.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Special Emphasis Panel (ZRG1-CB-N (30))
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Levy, Abraham
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University of California Santa Barbara
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Santa Barbara
United States
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Chen, Ming; Zakrewsky, Michael; Gupta, Vivek et al. (2014) Topical delivery of siRNA into skin using SPACE-peptide carriers. J Control Release 179:33-41
Oh, Seung Soo; Lee, Bongjae F; Leibfarth, Frank A et al. (2014) Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells. J Am Chem Soc 136:15010-5
Azarenko, Olga; Smiyun, Gregoriy; Mah, Jeffrey et al. (2014) Antiproliferative mechanism of action of the novel taxane cabazitaxel as compared with the parent compound docetaxel in MCF7 breast cancer cells. Mol Cancer Ther 13:2092-103
Thomas, Alexander W; Henson, Zachary B; Du, Jenny et al. (2014) Synthesis, characterization, and biological affinity of a near-infrared-emitting conjugated oligoelectrolyte. J Am Chem Soc 136:3736-9
Keeley, Patrick W; Luna, Gabriel; Fariss, Robert N et al. (2013) Development and plasticity of outer retinal circuitry following genetic removal of horizontal cells. J Neurosci 33:17847-62
Barua, Sutapa; Yoo, Jin-Wook; Kolhar, Poornima et al. (2013) Particle shape enhances specificity of antibody-displaying nanoparticles. Proc Natl Acad Sci U S A 110:3270-5