Funds are requested to purchase an Olympus Fluoview 1000S confocal instrument with state-of-the art functionality to replace an aging shared system that can no longer meet the research demands of our NIH funded researchers. There is a general demand for the increased speed and sensitivity that the Fluoview 1000S will provide. Additionally, collaborations across fields have broadened the research demands upon our facility and its shared confocal system. Our confocal system needs to support analyses of multiple organisms (e.g. marine invertebrates, C. elegans, and mammalian cell cultures), multiple probes (fluorescent proteins, quantum dots, and dyes) in multiple research paradigms. To provide this versatility, we have proposed a system with spectral imaging capabilities, a motorized X, Y stage and an environmental system that supports a range of imaging demands. This configuration will allow researchers to systematically sample large fields at high resolution, and to repeatedly sample multiple locations in fixed and live specimens. A replacement system is critical to insuring the on-going success of our NIH funded researchers and to supporting their biomedically relevant research programs.

Public Health Relevance

A number of NIH funded research projects depend on access to a state-of-the art confocal microscope. The replacement of an aging discontinued confocal microscope with the proposed state-of-the-art system will facilitate numerous biomedical research projects relevant to public health including projects on cancer, wound healing, polycystic kidney disease, diabetes and Alzheimer's disease.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Special Emphasis Panel (ZRG1-CB-N (30))
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Levy, Abraham
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University of California Santa Barbara
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Santa Barbara
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Benbow, Sarah J; Cook, Brett M; Reifert, Jack et al. (2016) Effects of Paclitaxel and Eribulin in Mouse Sciatic Nerve: A Microtubule-Based Rationale for the Differential Induction of Chemotherapy-Induced Peripheral Neuropathy. Neurotox Res 29:299-313
Camacho, Kathryn M; Menegatti, Stefano; Mitragotri, Samir (2016) Low-molecular-weight polymer-drug conjugates for synergistic anticancer activity of camptothecin and doxorubicin combinations. Nanomedicine (Lond) 11:1139-51
Cai, Danfeng; Dai, Wei; Prasad, Mohit et al. (2016) Modeling and analysis of collective cell migration in an in vivo three-dimensional environment. Proc Natl Acad Sci U S A 113:E2134-41
Langenbacher, Adam D; De Tomaso, Anthony W (2016) Temporally and spatially dynamic germ cell niches in Botryllus schlosseri revealed by expression of a TGF-beta family ligand and vasa. Evodevo 7:9
Clevenger, Tracy N; Hinman, Cassidy R; Ashley Rubin, Rebekah K et al. (2016) Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Eng Part A 22:597-609
Camacho, Kathryn M; Kumar, Sunny; Menegatti, Stefano et al. (2015) Synergistic antitumor activity of camptothecin-doxorubicin combinations and their conjugates with hyaluronic acid. J Control Release 210:198-207
Ramirez, M Desmond; Oakley, Todd H (2015) Eye-independent, light-activated chromatophore expansion (LACE) and expression of phototransduction genes in the skin of Octopus bimaculoides. J Exp Biol 218:1513-20
Bailey, Jeffrey K; Fields, Alexander T; Cheng, Kaijian et al. (2015) WD repeat-containing protein 5 (WDR5) localizes to the midbody and regulates abscission. J Biol Chem 290:8987-9001
Langenbacher, Adam D; Rodriguez, Delany; Di Maio, Alessandro et al. (2015) Whole-mount fluorescent in situ hybridization staining of the colonial tunicate Botryllus schlosseri. Genesis 53:194-201
Martinez Rodriguez, Nadine R; Das, Saurabh; Kaufman, Yair et al. (2015) Interfacial pH during mussel adhesive plaque formation. Biofouling 31:221-7

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