Funds are requested for the purchase of a Thermo LTQ-Orbitrap Elite mass spectrometer with electron transfer dissociation (ETD) capability. The requested instrument will be installed at, and managed by the W. M. Keck Proteomics Facility at the University of California Riverside. There are a core group of 14 NIH-funded investigators at UC Riverside who require extensive access to the proposed LTQ-Orbitrap Elite system for proteomic analysis. The progress of these research projects is currently hampered by the lack of a high- performance LC-MS/MS system for global proteome quantification, large-scale mapping of post-translational modifications, protein complex, and protein conformation studies. The availability of the proposed instrument will have an immediate and positive impact on the progress of these research projects. In particular, the high scan rate of the linear ion trap, together with the high mass-resolving power and high mass accuracy of the Orbitrap mass analyzer, provides capability in identifying and quantifying a large number of proteins with high confidence. In addition, the availability of electron transfer dissociation as an ion dissociation technique on the proposed instrument facilitates the unambiguous identifications of post-translational modifications with great accuracy. The availability of this state-of-the-art proteomis platform will further promote biomedical research at UC Riverside and enhance the research experience of students and post-docs from under-represented minority groups.

Public Health Relevance

The proposed LTQ-Orbitrap Elite mass spectrometer will benefit the research programs of a large group of NIH-funded investigators whose research requires the access of a high-performance mass spectrometer for proteomics research. The outcome of these research projects will improve our understanding of many human diseases including cancer, diabetes, and virus infection.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD010669-01A1
Application #
8447946
Study Section
Special Emphasis Panel (ZRG1-BCMB-D (30))
Program Officer
Birken, Steven
Project Start
2013-06-01
Project End
2014-05-31
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$600,000
Indirect Cost
Name
University of California Riverside
Department
Chemistry
Type
Schools of Earth Sciences/Natur
DUNS #
627797426
City
Riverside
State
CA
Country
United States
Zip Code
92521