This is an application by a group of 10 investigators to install a Zeiss LSM 780, a new generation confocal microscope suitable for multi-channel analysis of retinal circuitry and development. This model has "pig-tail" laser inputs to accommodate a variety of laser lines, high efficiency/low noise detectors and spectral analysis. These developments will make it possible to conduct 4 or 5 channel labeling experiments. The LSM 780 is self contained and menu-driven making it suitable for a multi-user facility. The instrument will be installed in the Confocal Module of the Vision Core Grant housed in the Department of Ophthalmology. This Vision Core facility is heavily used by 10 investigators conducting research on the visual system, including 8 supported by NEI, NIH programs. The service contract will be supported by the Ophthalmology department and there will be no usage charges for this group of investigators. The group is very well known for imaging studies of the retina with 6 recent cover pictures in peer-reviewed journals. In the broadest terms, we seek to understand the structure, function and development of the mammalian retina;such knowledge may lead to new strategies to alleviate visual disorders resulting from photoreceptor degeneration, glaucoma or diabetic retinopathy.

Public Health Relevance

The mammalian retina is our window to the visual world. It is a complex self-optimizing network built from approximately 60 types of neurons. We will use multi-channel imaging techniques to discover new cell types, to relate structure to function, to decode neural circuits and to follow the steps of retinal development. A sound base of such knowledge is essential to develop new strategies to alleviate retinal conditions leading to blindness.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD010768-01
Application #
8247570
Study Section
Special Emphasis Panel (ZRG1-CB-N (30))
Program Officer
Levy, Abraham
Project Start
2012-05-01
Project End
2013-10-31
Budget Start
2012-05-01
Budget End
2013-10-31
Support Year
1
Fiscal Year
2012
Total Cost
$543,362
Indirect Cost
Name
University of Texas Health Science Center Houston
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
800771594
City
Houston
State
TX
Country
United States
Zip Code
77225