Modern biochemistry requires the ability to quantitatively monitor biological reactions using a wide variety of reporters, including fluorophores and near-IR range dyes. In addition, radiolabels provide a very sensitive means of detections and have the advantage that they can be introduced without varying the chemical makeup of the sample. In this proposal, we request funds for the purchase of a Typhoon FLA9000, a multipurpose scanner that is capable of detecting all of these reporters in a variety of contexts, including polyacrylamide gels, multiwell plates, thin layer chromatography plates and others. This is a robust instrument that is highly suited for a multiuser facility. The associated software allows quantitation, background correction and export of the acquired data such that it can be analyzed in third party spreadsheet or graphing programs. The long term objectives of this proposal are to provide critical infrastructure to NIH-supported researchers. This instrument will replace a 10 year old Typhoon scanner that is outmoded and will soon be unrepairable. The new instrument will enhance capability by increasing the range of fluorophores and dyes that can be used to monitor biological reactions and providing improved sensitivity.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD012041-01
Application #
8247403
Study Section
Special Emphasis Panel (ZRG1-BST-F (30))
Program Officer
Levy, Abraham
Project Start
2012-05-15
Project End
2013-05-14
Budget Start
2012-05-15
Budget End
2013-05-14
Support Year
1
Fiscal Year
2012
Total Cost
$126,256
Indirect Cost
Name
Purdue University
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907