The Flow Cytometry Core Facility (FCCF) at the West Virginia University (WVU) School of Medicine is a centralized institutional shared research resource facility that broadly serves the research community at WVU and other regional institutions, and is operated in accordance with contemporary principles in management of institutional shared research facilities. The main priority of the FCCF is to support NIH- funded projects in biomedical research, including research funded with grants from NIGMS/NCRR""""""""s Center of Biomedical Research Excellence (CoBRE) and the Idea Network for Biomedical Research Excellence (INBRE) programs. Major instrumentation in the FCCF consists of a 2-laser Becton Dickinson (BD) FACSCalibur analytic flow cytometer, which was purchased with Institutional funds as part of WVU""""""""s commitment to biomedical research and has been in operation since 2002, and a 3-laser BD FACSAria cell sorter, which was purchased in 2006 with an NIH Shared Instrumentation Grant. The current proposal describes a plan to replace the FACSCalibur with an LSRFortessa. This upgrade will benefit the research community in 3 tangible ways. It will: 1) enable researchers to shift some of their analytic work from the capable but more expensive FACSAria cell sorter, thereby improving overall efficiency and reducing costs to individual investigators;2) improve the quality of the data being generated in the FCCF because of the advanced technology provided by the LSRFortessa;and 3) improve availability of analytic services in the FCCF because the FACSCalibur has, over the past 2 years, developed a history of chronic breakdowns despite diligent maintenance and careful oversight by FCCF scientists, as well as exhaustive efforts by Becton Dickinson service technicians.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD016165-01
Application #
8444190
Study Section
Special Emphasis Panel (ZRG1-CB-J (30))
Program Officer
Birken, Steven
Project Start
2013-06-01
Project End
2014-05-31
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$383,803
Indirect Cost
Name
West Virginia University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
191510239
City
Morgantown
State
WV
Country
United States
Zip Code
26506
Barbier, Mariette; Damron, F Heath (2016) Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling. PLoS One 11:e0146827
Slone, William L; Moses, Blake S; Hare, Ian et al. (2016) BCL6 modulation of acute lymphoblastic leukemia response to chemotherapy. Oncotarget 7:23439-53
Kozyreva, Varvara K; Kiseleva, Anna A; Ice, Ryan J et al. (2016) Combination of Eribulin and Aurora A Inhibitor MLN8237 Prevents Metastatic Colonization and Induces Cytotoxic Autophagy in Breast Cancer. Mol Cancer Ther 15:1809-22
Slone, William L; Moses, Blake S; Evans, Rebecca et al. (2016) Modeling Chemotherapy Resistant Leukemia In Vitro. J Vis Exp :e53645
Bachert, Beth A; Choi, Soo J; LaSala, Paul R et al. (2016) Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus. Front Cell Infect Microbiol 6:90
Armstead, Andrea L; Li, Bingyun (2016) Nanotoxicity: emerging concerns regarding nanomaterial safety and occupational hard metal (WC-Co) nanoparticle exposure. Int J Nanomedicine 11:6421-6433
Armstead, Andrea L; Li, Bingyun (2016) In vitro inflammatory effects of hard metal (WC-Co) nanoparticle exposure. Int J Nanomedicine 11:6195-6206
Wang, Kai; Bruce, Allison; Mezan, Ryan et al. (2016) Nanotopographical Modulation of Cell Function through Nuclear Deformation. ACS Appl Mater Interfaces 8:5082-92
Pifer, Phillip M; Farris, Joshua C; Thomas, Alyssa L et al. (2016) Grainyhead-like 2 inhibits the coactivator p300, suppressing tubulogenesis and the epithelial-mesenchymal transition. Mol Biol Cell 27:2479-92
Sisler, Jennifer D; Li, Ruibin; McKinney, Walter et al. (2016) Differential pulmonary effects of CoO and La2O3 metal oxide nanoparticle responses during aerosolized inhalation in mice. Part Fibre Toxicol 13:42

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