The objective of this proposal is to secure funding for a microcapillary LC-LTQ Orbitrap Elite mass spectrometer system to support shotgun proteomics-oriented, NIH-funded scientific goals of the greater Dartmouth biomedical research community. The users of the proposed system described in this application come from a broad range of departments and centers at Dartmouth College and Dartmouth Medical School, including the NCI-designated Norris Cotton Cancer Center (NCCC). Their projects represent a diverse continuum of research foci, from basic cellular mechanisms of cell cycle control and circadian rhythms to mechanisms of host-pathogen interactions and insulin signaling, to more translational projects aimed at understanding how cellular signaling is dysregulated in human lung cancers. Collectively, these research projects represent work and effort that has exceeded the capacity of the single LC-MS/MS platform that exists in all of Dartmouth for such experiments. Thus, the acquisition of a new mass spectrometer into the Dartmouth Proteomics Shared Resource is critically important to enable the completion of current, as well as future, NIH research goals of our faculty. The proposed instrumentation will be housed in our Proteomics Shared Resource, which is maintained by NCCC on behalf of the greater Dartmouth community.
The objective of this proposal is to facilitate existing and new biomedical research projects at Dartmouth College that rely on mass spectrometry-based shotgun proteomics. The impact from these projects has wide ranging benefits to human health, including insulin action in diabetes, how cells become re-wired in cancer, and how certain bacteria target lung cells of cystic fibrosis patients during chronic infections to exacerbate the disease.
|Cullati, Sierra N; Kabeche, Lilian; Kettenbach, Arminja N et al. (2017) A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase. J Cell Biol 216:2339-2354|
|Grassetti, Andrew V; Hards, Rufus; Gerber, Scott A (2017) Offline pentafluorophenyl (PFP)-RP prefractionation as an alternative to high-pH RP for comprehensive LC-MS/MS proteomics and phosphoproteomics. Anal Bioanal Chem 409:4615-4625|
|Petrone, Adam; Adamo, Mark E; Cheng, Chao et al. (2016) Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics. Mol Cell Proteomics 15:2448-61|
|Johnson, Michael E; Grassetti, Andrew V; Taroni, Jaclyn N et al. (2016) Stress granules and RNA processing bodies are novel autoantibody targets in systemic sclerosis. Arthritis Res Ther 18:27|
|Willger, S D; Liu, Z; Olarte, R A et al. (2015) Analysis of the Candida albicans Phosphoproteome. Eukaryot Cell 14:474-85|
|Rusin, Scott F; Schlosser, Kate A; Adamo, Mark E et al. (2015) Quantitative phosphoproteomics reveals new roles for the protein phosphatase PP6 in mitotic cells. Sci Signal 8:rs12|