This proposal requests $600,000 to purchase a CyTOF ICP-TOF-MS Model C5 mass cytometer with an automated 96 well plate reader and associated control computer made by DVS Sciences, Inc. The CyTOF is a new type of cytometer, which uses reagents labeled with metal ions instead of fluorescent dyes to detect different cellular constituents. Whereas practical flow cytometry techniques allow for only 10-12 simultaneous measurements, the CyTOF allows up to 100. The Investigators desiring to use this technology see great potential for advancement in personalized medicine as a more complete characterization of disease states and responses to various treatments is possible with the CyTOF compared to conventional flow cytometry. The CyTOF will be installed in the Stanford Shared FACS Facility, a Service Center in the School of Medicine that provides flow cytometry instruments and technical expertise to the Stanford and regional academic biotechnology research communities.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD016318-01
Application #
8448038
Study Section
Special Emphasis Panel (ZRG1-CB-J (30))
Program Officer
Birken, Steven
Project Start
2013-07-01
Project End
2014-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$600,000
Indirect Cost
Name
Stanford University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Angelo, Michael; Bendall, Sean C; Finck, Rachel et al. (2014) Multiplexed ion beam imaging of human breast tumors. Nat Med 20:436-42