This proposal requests funds to acquire a console and cold probe for a 600MHz NMR spectrometer to support biomolecular research at Weill Cornell Medical College. The instrument will directly support six NIH funded investigators and serve as a core resource for the medical college. Weill Cornell currently posses a single Varian 600 MHz spectrometer for high resolution heteronuclear protein NMR applications. Current utilization includes drug binding studies, PRE distance measurements, heteronuclear resonance assignments, protein structure determination and protein dynamics characterization. This is the only instrument at the university equipped for triple and quadruple resonance experiments, which is its primary mode of operation. The console and cold probe requested will replace13 year old electronics and provide a state of the art probe to better meet the demand for instrument time. This instrumentation will provide new capabilities including: enhanced signal-to-noise, improved salt tolerance, direct detection of 13C nuclei in addition to improved 1H sensitivity, and state-of-the-art NMR data collection and detection schemes. These features are currently unavailable on our older system. The instrumentation will positively impact basic and translational NIH funded research of relevance to cancer, neural growth/repair, diabetes, cardiovascular disease and amyloid pathogenesis.

Public Health Relevance

NMR spectrometers are essential for molecular structure determination and chemical identification. The NMR equipment will directly support NIH sponsored research aimed at understanding proteins involved in cancer, type-2 diabetes, Alzheimer's and Parkinson's diseases, cardiovascular problems, and anxiety and depression disorders. This equipment will be used to identify and characterize the proteins and peptides involved in these disease processes and will give detailed information about how these proteins work and provide pharmaceutical targets for treatment and prevention.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD016320-01
Application #
8447822
Study Section
Special Emphasis Panel (ZRG1-BCMB-D (30))
Program Officer
Levy, Abraham
Project Start
2013-09-01
Project End
2014-08-31
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
1
Fiscal Year
2013
Total Cost
$600,000
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
060217502
City
New York
State
NY
Country
United States
Zip Code
10065