As part of an established core facility, we request a Zeiss LSM780 laser-scanning confocal microscope with fluorescence correlation spectroscopy (FCS) on an inverted microscope stand. Addressing multiple live-cell/tissue studies, our needs come from three sources: oversubscription on existing laser-scanning confocal instruments, need to reduce photodamage to fluorescently labeled living cells, and need to resolve molecular-level events, particularly in living cells. Although we have three Zeiss laser- scanning confocals, they are heavily used, with peak usage 20h/day on one instrument. Photodamage and photobleaching are related to the instrument's signal-to-noise performance, and the LSM780 is outstanding in our tests, allowing techniques not possible with other confocals. To understand biological pathways, live-cell techniques become ever more important. Not only does FCS provide key molecular measurements about regulation of spatial organization within cells, it also discriminates hypotheses in signaling mechanisms in our systems. In addition, local efforts to construct new FRET biosensors and combine different biosensors into the same cells demand greater performance of instrumentation. These FRET studies are greatly facilitated by automated spectral unmixing. Ongoing live-cell studies use all these techniques, as well as FRAP, photoactivation, and basic time-lapse imaging. The proposed instrument will provide an order-of-magnitude superior performance that will enable key NIH- funded projects.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BST-T (30))
Program Officer
Levy, Abraham
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Johns Hopkins University
Anatomy/Cell Biology
Schools of Medicine
United States
Zip Code
Liu, Hsuan; Grantham, Michael L; Pekosz, Andrew (2018) Mutations in the Influenza A Virus M1 Protein Enhance Virus Budding To Complement Lethal Mutations in the M2 Cytoplasmic Tail. J Virol 92:
Merriman, Chengfeng; Huang, Qiong; Gu, Wei et al. (2018) A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic ?-cells. J Biol Chem 293:579-587
Zhu, Li; Kandasamy, Suresh K; Liao, Susan E et al. (2018) LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes. Nat Commun 9:4031
Lopez-Bertoni, Hernando; Kozielski, Kristen L; Rui, Yuan et al. (2018) Bioreducible Polymeric Nanoparticles Containing Multiplexed Cancer Stem Cell Regulating miRNAs Inhibit Glioblastoma Growth and Prolong Survival. Nano Lett 18:4086-4094
Wilson, David R; Sen, Rupashree; Sunshine, Joel C et al. (2018) Biodegradable STING agonist nanoparticles for enhanced cancer immunotherapy. Nanomedicine 14:237-246
West-Foyle, Hoku; Kothari, Priyanka; Osborne, Jonathan et al. (2018) 14-3-3 proteins tune non-muscle myosin II assembly. J Biol Chem 293:6751-6761
Zhang, Lei; Chang, Leslie; Xu, Jiajia et al. (2018) Frontal Bone Healing Is Sensitive to Wnt Signaling Inhibition via Lentiviral-Encoded Beta-Catenin Short Hairpin RNA. Tissue Eng Part A :
Merriman, Chengfeng; Li, Hua; Li, Huilin et al. (2018) Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8. J Biol Chem 293:16206-16216
Li, Xiaoguang; Edwards, Marc; Swaney, Kristen F et al. (2018) Mutually inhibitory Ras-PI(3,4)P2 feedback loops mediate cell migration. Proc Natl Acad Sci U S A 115:E9125-E9134
Pilarowski, Genay O; Vernon, Hilary J; Applegate, Carolyn D et al. (2018) Missense variants in the chromatin remodeler CHD1 are associated with neurodevelopmental disability. J Med Genet 55:561-566

Showing the most recent 10 out of 33 publications