This proposal requests funds for the purchase of a Nikon N-SIM/N-STORM Super-Resolution Microscope System. The instrument combines both SIM and STORM systems into one platform. The N-SIM (Structured Illumination Microscopy) achieves a resolution two times higher than that of conventional confocal microscopes in each dimension. It is equipped with 5 laser lines (458/488/515/532/561 nm) and capable of 3D imaging up to 20 ?m thickness. The N-STORM (Stochastic Optical Reconstruction Microscopy) is a single-molecule localization-based microscope that generates a dramatically enhanced resolution that is ten times (approx. 20 nm laterally) higher than confocal microscopy in all three axes. The N-STORM comes equipped with a 4-laser illumination unit (405/488/561/647 nm) and 3D STORM capability. Currently, there is no super-resolution microscope available at Stony Brook University. The system will facilitate the spatial resolution of protein localization and cellular structures beyond the diffraction limit of ~200nm, enabling investigators to explore fundamental biological problems related to human disease at high precision. Management and operation of the system will be provided by a technical specialist, who will also assists users in developing staining and imaging protocols for special projects, as well as helping investigators in the analysis of super-resolution data. )

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD016405-01
Application #
8447655
Study Section
Special Emphasis Panel (ZRG1-BST-T (30))
Program Officer
Levy, Abraham
Project Start
2013-06-20
Project End
2014-06-19
Budget Start
2013-06-20
Budget End
2014-06-19
Support Year
1
Fiscal Year
2013
Total Cost
$600,000
Indirect Cost
Name
State University New York Stony Brook
Department
Pharmacology
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794