The Cell and Molecular Imaging (CMI) Facility is a shared component of the Center for Cell Death, Injury and Regeneration (CCDIR) and the NCI-designated Hollings Cancer Center (HCC) whose purpose is to provide CCDIR/HCC investigators and other researchers at the Medical University of South Carolina (MUSC) with equipment and expertise for laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques. The only access of our investigators to combined confocal/mulitphoton microscopy is an 8+ year old Zeiss 510 NLO META microscope that is deficient in important functionalities that are essential for high performance confocal/multiphoton and intravital (in vivo) imaging, including spectral detection, long working distance water immersion optics and maximally sensitive descanned and nondescanned detection. Accordingly, the overall goal of this application is to upgrade the Zeiss 510 NLO microscope to an optimally configured and fully functional confocal/multiphoton microscope system to be made available to CCDIR, HCC and MUSC investigators. We propose to upgrade this system to a Zeiss 780 confocal/multiphoton inverted laser scanning microscope with maximally sensitive 3-channel non-descanned GaAsP detectors, a GaAsP-based 34-channel spectral array detector, 6 visible laser lines for confocal imaging, high performance long working distance intravital water-immersion optics and Zen software. Cost savings will be achieved by using microscope objectives, multiphoton laser, vibration isolation table and environmental chamber from the old microscope, whose remaining components will be traded in. The upgraded microscope system will be placed in a multiuser environment that provides technical support, user training and applications expertise in support of individual user projects.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD018113-01A1
Application #
8826419
Study Section
Special Emphasis Panel (ZRG1-CB-L (30))
Program Officer
Levy, Abraham
Project Start
2015-02-15
Project End
2016-02-14
Budget Start
2015-02-15
Budget End
2016-02-14
Support Year
1
Fiscal Year
2015
Total Cost
$570,473
Indirect Cost
Name
Medical University of South Carolina
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29403
Yang, Aimin; Qin, Shenghui; Schulte, Bradley A et al. (2017) MYC Inhibition Depletes Cancer Stem-like Cells in Triple-Negative Breast Cancer. Cancer Res 77:6641-6650
Sagar, Amin; Arif, Ehtesham; Solanki, Ashish Kumar et al. (2017) Targeting Neph1 and ZO-1 protein-protein interaction in podocytes prevents podocyte injury and preserves glomerular filtration function. Sci Rep 7:12047
Liu, Qinlong; Rehman, Hasibur; Krishnasamy, Yasodha et al. (2017) 8-pCPT-cGMP prevents mitochondrial depolarization and improves the outcome of steatotic partial liver transplantation. Int J Physiol Pathophysiol Pharmacol 9:69-83
Ghatak, Shibnath; Hascall, Vincent C; Markwald, Roger R et al. (2017) Transforming growth factor ?1 (TGF?1)-induced CD44V6-NOX4 signaling in pathogenesis of idiopathic pulmonary fibrosis. J Biol Chem 292:10490-10519
Grelet, Simon; Link, Laura A; Howley, Breege et al. (2017) A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. Nat Cell Biol 19:1105-1115
Farrand, Ariana Q; Helke, Kristi L; Gregory, Rebecca A et al. (2017) Vagus nerve stimulation improves locomotion and neuronal populations in a model of Parkinson's disease. Brain Stimul 10:1045-1054
He, Xiaoyuan; Yang, Aimin; McDonald, Daniel G et al. (2017) MiR-34a modulates ionizing radiation-induced senescence in lung cancer cells. Oncotarget 8:69797-69807
Lemasters, John J; Ramshesh, Venkat K; Lovelace, Gregory L et al. (2017) Compartmentation of Mitochondrial and Oxidative Metabolism in Growing Hair Follicles: A Ring of Fire. J Invest Dermatol 137:1434-1444
Hu, Jiangting; Kholmukhamedov, Andaleb; Lindsey, Christopher C et al. (2016) Translocation of iron from lysosomes to mitochondria during acetaminophen-induced hepatocellular injury: Protection by starch-desferal and minocycline. Free Radic Biol Med 97:418-426
Krishnasamy, Yasodha; Ramshesh, Venkat K; Gooz, Monika et al. (2016) Ethanol and High Cholesterol Diet Causes Severe Steatohepatitis and Early Liver Fibrosis in Mice. PLoS One 11:e0163342