Abstract

We are requesting funds for a Ventana Medical Systems Discovery XT(R) automated staining platform. The new instrument module will replace NEXes and Discovery modules that were damaged as a result of Superstorm Sandy. The NEXes platforms are no longer supported by the manufacturer and cannot be repaired. The Discovery has been evaluated and is scheduled for repairs. Due to the displacement of investigators and delays in research progress caused by the storm, many investigators are relying on our services for the collection of preliminary and project related IHC data. The increase in demand is significant and current outdated instrumentation cannot support this increase volume. The Discovery XT system automates immunohistochemical and in situ hybridization slide-based assays for the analysis of DNA, RNA and proteins in intact cells and tissue. The system will be placed in the Experimental Pathology Shared Resource Laboratory. The resource is administered by the Office of Collaborative Science and operated by an experienced and trained staff. The 8 NIH funded (1 DOD) users combined will use 98% of the instrument time.

Public Health Relevance

We are requesting funds for a Ventana Medical Systems Discovery XT(r) automated staining module to replace instrument modules that were damaged and are no longer operational as a result of Superstorm Sandy. The system automates immunohistochemical and in situ hybridization slide-based assays to enable the analysis of DNA, RNA and proteins in intact cells and tissue.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD018338-01
Application #
8704678
Study Section
Special Emphasis Panel (ZRG1-BCMB-P (30))
Program Officer
Levy, Abraham
Project Start
2014-02-17
Project End
2016-02-16
Budget Start
2014-02-17
Budget End
2016-02-16
Support Year
1
Fiscal Year
2014
Total Cost
$123,000
Indirect Cost

  Institution

Name
New York University
Department
Pathology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016

  Publications

Minton, Denise R; Nam, Minwoo; McLaughlin, Daniel J et al. (2018) Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs. Mol Cell 69:610-621.e5
Burgess, Hannah M; Pourchet, Aldo; Hajdu, Cristina H et al. (2018) Targeting Poxvirus Decapping Enzymes and mRNA Decay to Generate an Effective Oncolytic Virus. Mol Ther Oncolytics 8:71-81
Wang, Shiyang; Liechty, Benjamin; Patel, Seema et al. (2018) Programmed death ligand 1 expression and tumor infiltrating lymphocytes in neurofibromatosis type 1 and 2 associated tumors. J Neurooncol 138:183-190
Saint Fleur-Lominy, Shella; Maus, Mate; Vaeth, Martin et al. (2018) STIM1 and STIM2 Mediate Cancer-Induced Inflammation in T Cell Acute Lymphoblastic Leukemia. Cell Rep 24:3045-3060.e5
Winer, Benjamin Y; Shirvani-Dastgerdi, Elham; Bram, Yaron et al. (2018) Preclinical assessment of antiviral combination therapy in a genetically humanized mouse model for hepatitis delta virus infection. Sci Transl Med 10:
Martin, Patricia K; Marchiando, Amanda; Xu, Ruliang et al. (2018) Autophagy proteins suppress protective type I interferon signalling in response to the murine gut microbiota. Nat Microbiol 3:1131-1141
Fanok, Melania H; Sun, Amy; Fogli, Laura K et al. (2018) Role of Dysregulated Cytokine Signaling and Bacterial Triggers in the Pathogenesis of Cutaneous T-Cell Lymphoma. J Invest Dermatol 138:1116-1125
Snuderl, Matija; Kannan, Kasthuri; Pfaff, Elke et al. (2018) Recurrent homozygous deletion of DROSHA and microduplication of PDE4DIP in pineoblastoma. Nat Commun 9:2868
Xu, Mo; Pokrovskii, Maria; Ding, Yi et al. (2018) c-MAF-dependent regulatory T cells mediate immunological tolerance to a gut pathobiont. Nature 554:373-377
Gagner, Jean-Pierre; Sarfraz, Yasmeen; Ortenzi, Valerio et al. (2017) Multifaceted C-X-C Chemokine Receptor 4 (CXCR4) Inhibition Interferes with Anti-Vascular Endothelial Growth Factor Therapy-Induced Glioma Dissemination. Am J Pathol 187:2080-2094

Showing the most recent 10 out of 22 publications