This request is for funds to purchase a basic single photon Nikon A1 confocal microscope with two GaSP detectors. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optica, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Confocal microscopy is an essential service provided by the center, the current core use confocal microscopes within the facility are a 4 yr. old Nikon A1R, two Olympus FV1000's (8 and 10 years old) and a 14 year old Olympus fluoview 500, There is a single prime reason for this application: to provide critically needed instrument time to the NIH supported users of the Center for Biologic Imaging. Since the CBI commenced operations 22 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 300 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The PHS funded user base within the institution continues to expand, and a lack of available confocal time has become a major limiting factor in the utility of the center, this leads to considerable frustration amongst users. The acquisition of a basic single photon confocal microscope will be a tremendous addition to the activity and functionality of the center and will help alleviate the current user bottle neck for single photon confocal microscopy.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD019973-01
Application #
8825158
Study Section
Special Emphasis Panel (ZRG1-CB-L (30))
Program Officer
Levy, Abraham
Project Start
2015-04-01
Project End
2016-03-31
Budget Start
2015-04-01
Budget End
2016-03-31
Support Year
1
Fiscal Year
2015
Total Cost
$357,942
Indirect Cost
Name
University of Pittsburgh
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Chen, Yi-Jiun; Huang, Juan; Huang, Lynn et al. (2017) Phosphorylation potential of Drosophila E-Cadherin intracellular domain is essential for development and adherens junction biosynthetic dynamics regulation. Development 144:1242-1248
Klemens, Christine A; Edinger, Robert S; Kightlinger, Lindsay et al. (2017) Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem 292:375-385
Fitz, Nicholas F; Carter, Alexis Y; Tapias, Victor et al. (2017) ABCA1 Deficiency Affects Basal Cognitive Deficits and Dendritic Density in Mice. J Alzheimers Dis 56:1075-1085
Tohme, Samer; Kameneva, Marina V; Yazdani, Hamza O et al. (2017) Drag reducing polymers decrease hepatic injury and metastases after liver ischemia-reperfusion. Oncotarget 8:59854-59866
Ngo, Kevin; Patil, Prashanti; McGowan, Sara J et al. (2017) Senescent intervertebral disc cells exhibit perturbed matrix homeostasis phenotype. Mech Ageing Dev 166:16-23
Swan, Zachary D; Bouwer, Anthea L; Wonderlich, Elizabeth R et al. (2017) Persistent accumulation of gut macrophages with impaired phagocytic function correlates with SIV disease progression in macaques. Eur J Immunol 47:1925-1935
Dukes, April A; Bai, Qing; Van Laar, Victor S et al. (2016) Live imaging of mitochondrial dynamics in CNS dopaminergic neurons in vivo demonstrates early reversal of mitochondrial transport following MPP(+) exposure. Neurobiol Dis 95:238-49
Helfield, Brandon; Chen, Xucai; Watkins, Simon C et al. (2016) Biophysical insight into mechanisms of sonoporation. Proc Natl Acad Sci U S A 113:9983-8
Al Ghouleh, Imad; Meijles, Daniel N; Mutchler, Stephanie et al. (2016) Binding of EBP50 to Nox organizing subunit p47phox is pivotal to cellular reactive species generation and altered vascular phenotype. Proc Natl Acad Sci U S A 113:E5308-17
Tohme, Samer; Yazdani, Hamza O; Al-Khafaji, Ahmed B et al. (2016) Neutrophil Extracellular Traps Promote the Development and Progression of Liver Metastases after Surgical Stress. Cancer Res 76:1367-80

Showing the most recent 10 out of 17 publications