This proposal requests funds for acquisition and continuing operation of a two-dimensional ultra performance liquid chromatography coupled with a high resolution and high accuracy mass spectrometer with tandem MS/MS capability (2DLC-MS/MS) for metabolomics. This instrument will be located in the Shumaker Research Building (room 335) at the Center for Regulatory Environmental and Analytical Metabolomics (CREAM), as a part of integrated metabolomics platforms to support the basic, translational and clinical research at the University of Louisville. The proposed instrument will complement and gradually replace our use of the existing hybrid quadrupole linear ion-trap and Fourier transform ion cyclotron resonance mass spectrometry (LTQ- FTICR-MS) instrument. This LTQ-FTICR-MS has been formally designated as in retired status by the manufacturer as of January 2014 and the manufacturer will now only provide preventive maintenance. We are under risk, at any moment, of losing the capability of conducting high resolution high accuracy mass spectrometry-based metabolomics analyses at the CREAM Center.
The Specific Aims of this proposal are provision of the following for the University of Louisville biomedical research community:
Specific Aim 1 : For the established Major Users (78% allotment of time): Provision of increased capacity for identification and quantification of metabolites with complementary analyses of spatial and temporal flux through metabolic pathways.
Specific Aim 2 : For appropriate Research Centers & Institutes, less frequent (<100 samples / year) Users, and prospective Users of mass spectrometry (17% allotment of time): Vigorous promotion of the use of high accuracy and high resolution mass spectrometry in biomedical research with an education program for research and scientific career development.

Public Health Relevance

This proposal requests funds for purchase of a LC-MS system that couples an ultra performance liquid chromatography with a high resolution and high accuracy mass spectrometer with tandem MS/MS capability (2DLC-MS/MS). The 2DLC-MS/MS instrument will be located at the CREAM Center as a part of integrated analytical platforms to provide metabolomics service for the biomedical research community at the University of Louisville.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD020106-01
Application #
8826389
Study Section
Special Emphasis Panel (ZRG1-BCMB-D (30))
Program Officer
Levy, Abraham
Project Start
2015-02-27
Project End
2016-02-26
Budget Start
2015-02-27
Budget End
2016-02-26
Support Year
1
Fiscal Year
2015
Total Cost
$600,000
Indirect Cost
Name
University of Louisville
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40202