We request funds for a Thermo Fisher Scientific Orbitrap Fusion mass spectrometer with ETD (Electron Transfer Dissociation) to be housed in the proteomics core of the UC Davis Genome center. The core facility is the sole resource in the area and performs a large number of proteomic analyses. However, the needs of many researchers in the areas of post-translational modifications, quantitation, and top-down analyses are currently not being met with existing instrumentation. The Thermo Scientific Fusion ETD is a next generation state-of-the-art hybrid mass spectrometer that has several unique characteristics that will enable the proteomics core to characterize endogenous post-translational modification including phosphorylation, glycosylation, and chemical modifications with cross-linking agents. It will also enable the comprehensive structural elucidation of large macropeptides and proteins. It will further enhance our capabilities for quantitating proteins. There is currently an unmet demand by the core group of NIH funded investigators for proteomic analyses that includes quantitative proteomic profiling, site-specific analysis of endogenous post-translational modifications such phosphorylation and O- and N-linked glycosylation, and chemical modification such as in cross linking experiments. There is a need for de novo sequencing of large macropeptides with extensive post translational modifications. The Orbitrap Fusion ETD is expected to have an immediate and a major impact on the research efforts of the major users listed in this proposal and the greater UC Davis research community and the nascent biotechnology start-ups clustered in the areas. This mass spectrometer will be integrated into our core facility and its existing infrastructure will integrate the operation, maintenance and management of this instrument.

Public Health Relevance

The acquisition of a Thermo Fusion mass spectrometer with ETD is requested to provide new capabilities for analyzing proteins with extensive post-translation modification. It will impact research in several public health-related topics including AIDS associated fungal infections, human milk-gut microbe interactions, and autoimmune diseases.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD021801-01
Application #
9076052
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Levy, Abraham
Project Start
2016-04-15
Project End
2017-04-14
Budget Start
2016-04-15
Budget End
2017-04-14
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
University-Wide
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618