The Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cancer Genomics Core Laboratory, an NCI-supported Shared Resource is requesting funds to purchase a Perkin Elmer Sciclone G3 NGSx Workstation from Perkin Elmer. This workstation is an automated platform designed to prepare libraries for Next Generation DNA Sequencing (NGS) platforms. The instrument is flexible in design and has the capability to prepare 8-96 libraries per run. This Sciclone G3 NGSx Workstation prepares libraries for all NGS applications including RNAseq, ChIPseq, DNAseq, exome sequencing, and small RNAseq, and comes with preset programs for NGS libraries preparation from multiple vendors. The workstation has a user friendly programmable power controller that can be programmed to implement new NGS library protocols as they are developed. The instrument will be located in the Cancer Genomics Core Laboratory, which is supported by the WFBCCC Shared Resource Grant. The workstation will support 10 Major Users with NIH funding and 6 Minor Users with NIH grants across multiple departments at Wake Forest University Health Sciences (WFUHS). This instrumentation grant has outstanding support from the WFUHS faculty and outstanding financial support from the WFUHS and WFBCCC department chairs and administration

Public Health Relevance

The Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cancer Genomics Core Laboratory provides Next Generation DNA sequencing (NGS) services the Wake Forest University Health Sciences research community. The NGS services have been rapidly growing over the past year and automation is needed to process the NGS libraries for DNA sequencing. This proposal is requesting funding for a Perkin Elmer Sciclone G3 NGSx Workstation to support automated NGS library process in the Cancer Genomics Core laboratory.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD023409-01
Application #
9272640
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Levy, Abraham
Project Start
2017-04-10
Project End
2018-04-09
Budget Start
2017-04-10
Budget End
2018-04-09
Support Year
1
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157