An IVIS(R) Spectrum Imaging system (Caliper Life Sciences, Inc.) is requested and will be set up as a shared instrument core facility at the University of Rochester Medical Center (URMC). Imaging technologies that allow for unmixed data acquisition at different spectral bands are increasingly useful in biomedical research The requested IVIS Spectrum has unique capabilities for sensitively imaging both bioluminescent and fluorescent reporters within the same animal without mixing the multiple spectra. The system performs both epi- and trans-illumination fluorescent imaging and uses high efficiency narrow band- pass filters coupled with spectral unmixing algorithms to differentiate between multiple shallow and deep fluorescent sources. This is useful in optimally enabling a wide array of fluorescence/bioluminescence research applications. The system is also capable of performing 3D tomographic reconstructions for BOTH bioluminescence and fluorescence, and enables data co-registration with complementary modalities such as CT and MRI. The shared instrument will compliment and boost the extensive imaging resources available to URSMD researchers and will enable new research directions that will ultimately enhance the prospects of making breakthrough medical discoveries and attracting substantial research grants funding. This transformative technology will also allow URSMD researchers to perform intra-subject, longitudinal, in vivo imaging and data acquisition with significantly smaller sample sizes, which will significantly reduce the cost of research and improve statistical power of the studies utilizing this instrument. The User's pool identified in this grant application draws a dozen NIH-funded investigators from 11 different URSMD departments pursuing wide ranging areas of biomedical research with emphasis on clinical translational areas that are well-aligned with the mission of the URSMD's Clinical and Translational Sciences Institute (CTSI). These projects include studying various disease models and development of therapeutics, dissecting cellular and molecular signaling mechanisms in vivo and in real time, and tissue engineering and regenerative medicine, to name a few. The URSMD has made an unequivocal commitment to the provision of space and resources towards the establishment of the proposed Molecular Multispectral Imaging facility, which will help generate short-term and long-term employment opportunities that are consistent with the goals of the American Recovery and Reinvestment Act (ARRA). PUBLIC HEALTH RELEVACNE: Funding for an IVIS(r) Spectrum Multimodal (multispectral bioluminescence and fluorescence) Imaging instrument is requested. The shared instrument will compliment and boost the extensive imaging resources available to URSMD researchers engaged in wide ranging areas of biomedical research with emphasis on clinical translation in alignment with the mission of the URSMD's Clinical and Translational Sciences Institute (CTSI).

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Special Emphasis Panel (ZRG1-SBIB-D (30))
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Birken, Steven
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University of Rochester
Schools of Dentistry
United States
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Yang, Li; Shen, MingJing; Xu, Li Jun et al. (2017) Enhancing NK cell-mediated cytotoxicity to cisplatin-resistant lung cancer cells via MEK/Erk signaling inhibition. Sci Rep 7:7958
Wang, Yuchen; Malcolm, Dominic W; Benoit, Danielle S W (2017) Controlled and sustained delivery of siRNA/NPs from hydrogels expedites bone fracture healing. Biomaterials 139:127-138
Wang, Yuchen; Newman, Maureen R; Ackun-Farmmer, Marian et al. (2017) Fracture-Targeted Delivery of ?-Catenin Agonists via Peptide-Functionalized Nanoparticles Augments Fracture Healing. ACS Nano 11:9445-9458
Shen, Ming Jing; Xu, Li Jun; Yang, Li et al. (2017) Radiation alters PD-L1/NKG2D ligand levels in lung cancer cells and leads to immune escape from NK cell cytotoxicity via IL-6-MEK/Erk signaling pathway. Oncotarget 8:80506-80520
de Mesy Bentley, Karen L; Trombetta, Ryan; Nishitani, Kohei et al. (2017) Evidence of Staphylococcus Aureus Deformation, Proliferation, and Migration in Canaliculi of Live Cortical Bone in Murine Models of Osteomyelitis. J Bone Miner Res 32:985-990
Chen, Amanda X; Hoffman, Michael D; Chen, Caressa S et al. (2015) Disruption of cell-cell contact-mediated notch signaling via hydrogel encapsulation reduces mesenchymal stem cell chondrogenic potential: winner of the Society for Biomaterials Student Award in the Undergraduate Category, Charlotte, NC, April 15 to 18, 20 J Biomed Mater Res A 103:1291-302
Nishitani, Kohei; Sutipornpalangkul, Werasak; de Mesy Bentley, Karen L et al. (2015) Quantifying the natural history of biofilm formation in vivo during the establishment of chronic implant-associated Staphylococcus aureus osteomyelitis in mice to identify critical pathogen and host factors. J Orthop Res 33:1311-9
Hoffman, Michael D; Benoit, Danielle S W (2015) Emulating native periosteum cell population and subsequent paracrine factor production to promote tissue engineered periosteum-mediated allograft healing. Biomaterials 52:426-40
Varrone, John J; de Mesy Bentley, Karen L; Bello-Irizarry, Sheila N et al. (2014) Passive immunization with anti-glucosaminidase monoclonal antibodies protects mice from implant-associated osteomyelitis by mediating opsonophagocytosis of Staphylococcus aureus megaclusters. J Orthop Res 32:1389-96
Hoffman, Michael D; Van Hove, Amy H; Benoit, Danielle S W (2014) Degradable hydrogels for spatiotemporal control of mesenchymal stem cells localized at decellularized bone allografts. Acta Biomater 10:3431-41

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