We request funds to purchase an integrated system for liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS). The system includes a Applied Biosystems (ABI) QStar Elite quadrupole time-of-flight (QqTOF) mass spectrometer capable of rapid scanning and high accuracy mass measurements, and a Waters ultra-high pressure liquid chromatography system for high resolution analyte separations. The proposed instrument will replace a current ABI QStar Pulsar QqTOF mass spectrometer, which must be retired on July 1, 2010, when ABI terminates all repair and service of the current instrument. The system represents a key instrument at the Central Analytical Facility, which serves investigators across the University of Colorado at Boulder (CU-Boulder) campus. The current QqTOF instrument is our workhorse for service samples analyzed by the facility and is currently used by more than 20 laboratories across this campus. It is most heavily used to support research in synthetic organic chemistry and materials development, including structural and elemental analysis of small molecules, natural products, and polymers, and for negative and positive ionization mode analysis of synthetic nucleotides and nucleic acids. It is also used extensively for proteomics applications, including hydrogen-deuterium exchange measurements to map protein-protein and protein-ligand interfaces, and routine ESI-LC-MS/MS to carry out peptide sequencing of protein digests in order to determine subunit compositions of protein complexes. The current QqTOF instrument is used nearly 24/7, and is an essential component of the Central Analytical Facility. Its replacement with the QStar Elite QqTOF is critically needed to continue supporting a wide range of research needs at CU-Boulder.

Public Health Relevance

Funding is requested to purchase a new mass spectrometry system, to replace an existing system that will be retired in 2010, when the manufacturer discontinues repair and service. This is a key analytical technology for """"""""weighing"""""""" molecules, which is essential for biomedical research because it allows the chemical composition of molecules to be defined. The instrument will be shared by more than 20 laboratories at the University of Colorado at Boulder, who are synthesizing new materials or characterizing proteins, with the goal of understanding how to diagnose and treat human diseases.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR026641-01
Application #
7792846
Study Section
Special Emphasis Panel (ZRG1-BCMB-R (30))
Program Officer
Levy, Abraham
Project Start
2010-08-01
Project End
2011-07-31
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
1
Fiscal Year
2010
Total Cost
$458,406
Indirect Cost
Name
University of Colorado at Boulder
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309
Walder, Robert; LeBlanc, Marc-André; Van Patten, William J et al. (2017) Rapid Characterization of a Mechanically Labile ?-Helical Protein Enabled by Efficient Site-Specific Bioconjugation. J Am Chem Soc 139:9867-9875
Rapf, Rebecca J; Perkins, Russell J; Yang, Haishen et al. (2017) Photochemical Synthesis of Oligomeric Amphiphiles from Alkyl Oxoacids in Aqueous Environments. J Am Chem Soc 139:6946-6959
Mattiroli, Francesca; Gu, Yajie; Yadav, Tejas et al. (2017) DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication. Elife 6:
Mattiroli, Francesca; Gu, Yajie; Balsbaugh, Jeremy L et al. (2017) The Cac2 subunit is essential for productive histone binding and nucleosome assembly in CAF-1. Sci Rep 7:46274
Liu, Wallace H; Roemer, Sarah C; Zhou, Yeyun et al. (2016) The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones. Elife 5:
Chattaraj, Rajarshi; Mohan, Praveena; Livingston, Clare M et al. (2016) Mutually-Reactive, Fluorogenic Hydrocyanine/Quinone Reporter Pairs for In-Solution Biosensing via Nanodroplet Association. ACS Appl Mater Interfaces 8:802-8