We are applying for funds to replace the ThermoFisher single quadrupole Trace MS GC mass spectrometer that was installed in 1999 and that is used to quantify small volatile lipids. In the last year about 47% of all analyses were performed on the Trace MS. Improvements in electronics, computers, and software have put the capabilities of this instrument several generations behind and we have several users that need the sensitivity and capabilities routinely available on newer tandem MS/MS instruments. We have chosen to replace the Trace MS with a triple quadrupole tandem mass spectrometer, the TSQ Quantum GC from ThermoFisher Corporation. Detection sensitivity and reliability were the principal factors in our evaluation. The TSQ Quantum GC interfaced to a Trace GC will be installed in the Mass Spectrometer Facility located at Wake Forest University School of Medicine (WFUSM). Three mass spectrometers that specialize in different types of analyses are located in the Mass Spectrometer Facility. Peptide sequencing is performed using a Waters Q-TOF API US tandem mass spectrometer interfaced to a Waters CapLC. Routine phospholipid analysis is performed on the TSQ Discovery Max triple quadrupole mass spectrometer interfaced with an updated Agilent 1100 HPLC. The TSQ Quantum GC MS/MS will replace the Trace MS that is used for the analysis of small, volatile lipids. The Facility webpage is at http://www1.wfubmc.edu/mass_spec/. There are three individuals associated with the Facility. The Facility Director, Dr. Michael Thomas, helps users design and implement experiments and then helps them interpret the results. Two technicians are available to run sample analyses. One of the technicians, Dr. John Owen, is well versed in sample preparation and will work up samples for investigators or help them design sample work up protocols. Students and postdoctoral fellows are encouraged to use the equipment after appropriate training. The Mass Spectrometer Facility has offered mass spectrometric resources to WFUSM investigators since 1981. Yearly use of the facility has consistently increased over the past 25 years. During the past three years the facility served 36 WFUSM investigators and their collaborators from 11 departments and 6 academic investigators not associated with WFUSM. The Comprehensive Cancer Center of Wake Forest University (CCCWFU), fee-for-service and the Institution are committed to support the facility. In Fiscal 2008 the cost of the facility was $166,816 with 57% covered by fee-for-service and 43% covered by CCCWFU. There was no out-of-pocket cost to the Institution. This Facility supports grants with a combined value of more than $13M. Because of the heavy usage of the Trace MS by WFUSM faculty and students it is essential to maintain a mass spectrometer that operates with the best sensitivity and resolution that is commercially available.

Public Health Relevance

The Mass Spectrometer Facility supports NIH-funded research at Wake Forest University and GC/MS capability is an essential part of that support. Replacing our GC/MS with a new instrument having added capabilities will enhance the timely completion of NIH-funded research.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR027940-01
Application #
7796385
Study Section
Special Emphasis Panel (ZRG1-BCMB-R (30))
Program Officer
Birken, Steven
Project Start
2010-04-22
Project End
2011-04-21
Budget Start
2010-04-22
Budget End
2011-04-21
Support Year
1
Fiscal Year
2010
Total Cost
$208,705
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Biochemistry
Type
Schools of Medicine
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Qi, Bowen; Crawford, Ayrianne J; Wojtynek, Nicholas E et al. (2018) Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer. Nanomedicine 14:769-780
Payne, William M; Svechkarev, Denis; Kyrychenko, Alexander et al. (2018) The role of hydrophobic modification on hyaluronic acid dynamics and self-assembly. Carbohydr Polym 182:132-141
Svechkarev, Denis; Kyrychenko, Alexander; Payne, William M et al. (2018) Probing the self-assembly dynamics and internal structure of amphiphilic hyaluronic acid conjugates by fluorescence spectroscopy and molecular dynamics simulations. Soft Matter 14:4762-4771
Svechkarev, Denis; Sadykov, Marat R; Bayles, Kenneth W et al. (2018) Ratiometric Fluorescent Sensor Array as a Versatile Tool for Bacterial Pathogen Identification and Analysis. ACS Sens 3:700-708
Bhattacharya, D; Svechkarev, D; Souchek, J J et al. (2017) Impact of structurally modifying hyaluronic acid on CD44 interaction. J Mater Chem B 5:8183-8192
Hill, Tanner K; Davis, Amanda L; Wheeler, Frances B et al. (2016) Development of a Self-Assembled Nanoparticle Formulation of Orlistat, Nano-ORL, with Increased Cytotoxicity against Human Tumor Cell Lines. Mol Pharm 13:720-8
Hill, Tanner K; Kelkar, Sneha S; Wojtynek, Nicholas E et al. (2016) Near Infrared Fluorescent Nanoparticles Derived from Hyaluronic Acid Improve Tumor Contrast for Image-Guided Surgery. Theranostics 6:2314-2328
Pollard, Ricquita D; Blesso, Christopher N; Zabalawi, Manal et al. (2015) Procollagen C-endopeptidase Enhancer Protein 2 (PCPE2) Reduces Atherosclerosis in Mice by Enhancing Scavenger Receptor Class B1 (SR-BI)-mediated High-density Lipoprotein (HDL)-Cholesteryl Ester Uptake. J Biol Chem 290:15496-511
Black, Leland L; Srivastava, Roshni; Schoeb, Trenton R et al. (2015) Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice. J Immunol 195:4685-98
Hill, Tanner K; Abdulahad, Asem; Kelkar, Sneha S et al. (2015) Indocyanine green-loaded nanoparticles for image-guided tumor surgery. Bioconjug Chem 26:294-303

Showing the most recent 10 out of 15 publications