Protein misfolding and abnormal aggregation is known to be characteristic of many diseases and often coincident with peptide mutations. Our long-term goal is to understand the effects of peptide mutations upon the relative energies of the various peptide secondary structures and how they are related to these diseases, with particular emphas placed on diseases related to collagen structure and Alzheimer's disease. With respect to the collagen related diseases, we suggest that these mutations cause errors in the H-bonding patterns of normal collagen. With respect to Alzheimer's disease, we suspect that mutations affect the relative amounts and positions of local alpha-helical structure in the protein, tau, which can change its propensity to be phosphoylated. Hyperphosphorylation has been directly related to the abnormal aggregation of tau which is a physiological symptom of Alzheimer's Disease.
The specific aims of the proposed work will be the elucidation the structural factors that determine that determine the secondary structures of peptides using density functional and semi empirical molecular orbital theory, both in isolated (gas phase) and solvated completely geometrically optimized oligopeptides. This work will be coordinated with NMR structural determinations of the same peptides where possible.
A second aim will be the application of these and other similar energetic studies to the improved understanding of the details of several diseases that involve mutated, misfolded and/or unnaturally aggregated peptides and proteins. The specific foci of these studies will be collagen-like triple helices (related to osteogenesis imperfecta, Ehlers-Danlos syndrome, Alport syndrome, Schmid metaphyseal chondrodysplasia and dystrophic epidermolysis bullosa) and the protein tau (involved in Alzheimer's disease). First principles (quantum mechanical) calculations will be used to determine the relative energies of the secondary structures of different peptides. The knowledge gleaned from these studies will be applied to determining the properties misfolded proteins that cause several diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Enhancement Award (SC1)
Project #
5SC1AG034197-04
Application #
8223202
Study Section
Special Emphasis Panel (ZGM1-MBRS-X (CH))
Program Officer
Velazquez, Jose M
Project Start
2009-01-15
Project End
2013-04-30
Budget Start
2012-02-15
Budget End
2013-04-30
Support Year
4
Fiscal Year
2012
Total Cost
$253,123
Indirect Cost
$86,595
Name
Hunter College
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
620127915
City
New York
State
NY
Country
United States
Zip Code
10065
Plumley, Joshua A; Ali-Torres, Jorge; Pohl, Gabor et al. (2014) Capping amyloid ?-sheets of the tau-amyloid structure VQIVYK with hexapeptides designed to arrest growth. An ONIOM and density functional theory study. J Phys Chem B 118:3326-34
Pohl, Gabor; Asensio, Amparo; Dannenberg, J J (2014) Capping parallel ?-sheets of acetyl(Ala)6NH2 with an acetyl(Ala)5ProNH2 can arrest the growth of the sheet, suggesting a potential for curtailing amyloid growth. An ONIOM and density functional theory study. Biochemistry 53:617-23
Pohl, Gabor; Plumley, Joshua A; Dannenberg, J J (2013) The interactions of phenylalanines in *-sheet-like structures from molecular orbital calculations using density functional theory (DFT), MP2, and CCSD(T) methods. J Chem Phys 138:245102
Marianski, Mateusz; Dannenberg, J J (2012) Aqueous solvation of polyalanine *-helices with specific water molecules and with the CPCM and SM5.2 aqueous continuum models using density functional theory. J Phys Chem B 116:1437-45
Plumley, Joshua A; Tsai, Midas I-Hsien; Dannenberg, J J (2011) Aggregation of capped hexaglycine strands into hydrogen-bonding motifs representative of pleated and rippled ýý-sheets, collagen, and polyglycine I and II crystal structures. A density functional theory study. J Phys Chem B 115:1562-70
Roy, Dipankar; Dannenberg, J J (2011) The Effects of Regularly Spaced Glutamine Substitutions on Alpha-Helical Peptide Structures. A DFT/ONIOM Study. Chem Phys Lett 512:255-257
Plumley, Joshua A; Dannenberg, J J (2011) A comparison of the behavior of functional/basis set combinations for hydrogen-bonding in the water dimer with emphasis on basis set superposition error. J Comput Chem 32:1519-27
Salvador, Pedro; Tsai, I-Hsien Midas; Dannenberg, J J (2011) J-coupling constants for a trialanine peptide as a function of dihedral angles calculated by density functional theory over the full Ramachandran space. Phys Chem Chem Phys 13:17484-93
Plumley, Joshua A; Dannenberg, J J (2011) Comparison of ?-sheets of capped polyalanine with those of the tau-amyloid structures VQIVYK and VQIINK. A density functional theory study. J Phys Chem B 115:10560-6
Plumley, Joshua A; Dannenberg, J J (2010) The importance of hydrogen bonding between the glutamine side chains to the formation of amyloid VQIVYK parallel beta-sheets: an ONIOM DFT/AM1 study. J Am Chem Soc 132:1758-9