Mosquito gut microbiome is critical for priming mosquito basal immunity. As an effort to understand microbiome in the mosquito gut ecosystem, this project addresses important questions regarding how non-pathogenic bacteria colonize the gut and interact with host immune surveillance to maintain a symbiotic relationship, which in turn provides effective protection against pathogens like malaria. The bacterial cell wall component lipopolysaccharide (LPS) makes a connection of colonization and immunogenicity. We have isolated a predominant gut bacterium Enterobacter sp., from the mosquito gut, and a mutant was generated, in which the gene waaL was disrupted. The LPS of the mutant strain has defects. We will use the wild type and the waal mutant to study the role of LPS in bacterial colonization of the gut and its role in immunogenic potency. We will create an in vivo working model using GFP- or RFP- tagged Enterobacter wild type or mutant to reconstruct the gut population, and then determine its efficiency of colonization. The RNA-seq will be used for analyzing transcriptomes of both Enterobacter and the gut simultaneously, a dual approach to acquire data pertinent to the mutual interactions between the bacteria and host. The impact of the LPS defects on bacterial immunogenicity and host immunity against malaria will also be assessed. The project will generate data to advance our understanding how a symbiotic microbial community is shaped, and how host immune system interacts with co-evolved non-pathogenic microorganisms to maintain symbiostasis in the gut ecosystem. The knowledge will facilitate to develop novel ways to strengthen mosquito anti-malaria immunity to reduce malaria transmission.

Public Health Relevance

This project addresses the role of bacterial cell wall component LPS in bacterial colonization and immunogenicity in mosquito gut ecosystem. The impact of bacterial community on malaria competence will be evaluated.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Enhancement Award (SC1)
Project #
1SC1AI112786-01
Application #
8630774
Study Section
Special Emphasis Panel (ZGM1-TWD-9 (SC))
Program Officer
Costero, Adriana
Project Start
2014-04-01
Project End
2018-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
1
Fiscal Year
2014
Total Cost
$308,651
Indirect Cost
$88,651
Name
New Mexico State University Las Cruces
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
173851965
City
Las Cruces
State
NM
Country
United States
Zip Code
88003
Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2014) Insights from the genome annotation of Elizabethkingia anophelis from the malaria vector Anopheles gambiae. PLoS One 9:e97715