Mosquito gut microbiome is critical for priming mosquito basal immunity. As an effort to understand microbiome in the mosquito gut ecosystem, this project addresses important questions regarding how non-pathogenic bacteria colonize the gut and interact with host immune surveillance to maintain a symbiotic relationship, which in turn provides effective protection against pathogens like malaria. The bacterial cell wall component lipopolysaccharide (LPS) makes a connection of colonization and immunogenicity. We have isolated a predominant gut bacterium Enterobacter sp., from the mosquito gut, and a mutant was generated, in which the gene waaL was disrupted. The LPS of the mutant strain has defects. We will use the wild type and the waal mutant to study the role of LPS in bacterial colonization of the gut and its role in immunogenic potency. We will create an in vivo working model using GFP- or RFP- tagged Enterobacter wild type or mutant to reconstruct the gut population, and then determine its efficiency of colonization. The RNA-seq will be used for analyzing transcriptomes of both Enterobacter and the gut simultaneously, a dual approach to acquire data pertinent to the mutual interactions between the bacteria and host. The impact of the LPS defects on bacterial immunogenicity and host immunity against malaria will also be assessed. The project will generate data to advance our understanding how a symbiotic microbial community is shaped, and how host immune system interacts with co-evolved non-pathogenic microorganisms to maintain symbiostasis in the gut ecosystem. The knowledge will facilitate to develop novel ways to strengthen mosquito anti-malaria immunity to reduce malaria transmission.
This project addresses the role of bacterial cell wall component LPS in bacterial colonization and immunogenicity in mosquito gut ecosystem. The impact of bacterial community on malaria competence will be evaluated.
|Champion, Cody J; Xu, Jiannong (2016) The impact of metagenomic interplay on the mosquito redox homeostasis. Free Radic Biol Med :|
|Chen, Huiying; Li, Kaili; Shi, Hua et al. (2016) Ecological niches and blood sources of sand fly in an endemic focus of visceral leishmaniasis in Jiuzhaigou, Sichuan, China. Infect Dis Poverty 5:33|
|Wang, Yan; Yu, Wanqin; Shi, Hua et al. (2015) Historical survey of the kdr mutations in the populations of Anopheles sinensis in China in 1996-2014. Malar J 14:120|
|Pei, Dong; Hill-Clemons, Casey; Carissimo, Guillaume et al. (2015) Draft Genome Sequences of Two Strains of Serratia spp. from the Midgut of the Malaria Mosquito Anopheles gambiae. Genome Announc 3:|
|Pei, Dong; Jiang, Jinjin; Yu, Wanqin et al. (2015) The waaL gene mutation compromised the inhabitation of Enterobacter sp. Ag1 in the mosquito gut environment. Parasit Vectors 8:437|
|Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2014) Insights from the genome annotation of Elizabethkingia anophelis from the malaria vector Anopheles gambiae. PLoS One 9:e97715|
|Wang, Yan; Xu, Jiannong; Ma, Yajun (2014) Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China. Parasit Vectors 7:592|
|Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2013) Draft Genome Sequences of Elizabethkingia anophelis Strains R26T and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae. Genome Announc 1:|