Malaria vector competence is largely determined by ecological interactions among mosquito gut microbiota, Plasmodium, and host immunity in the context of gut microenvironment. Mosquito immunity studies have been mainly focusing on the adults. Larva-pathogen interaction is an unignorable driving force in shaping mosquito immune system during the evolution. However, little is known about larva immune structure and its interaction with gut microbial flora. In this project we propose to use larvicidal Bacillus sphaericus to study mosquito larval immune response, and characterize larval immune architecture by using microarray based global expression profiling. The impact of larval immune activation on the dynamics of gut microbiota in both larvae and adults will be assessed by profiling gut bacterial community via high throughput pyrosequencing bacterial 16S rDNA marker. The influence of these parameters on the malaria susceptibility will also be evaluated. The project will generate data towards a better understanding of larval immunity and its impact on mosquito gut ecosystem.

Public Health Relevance

Mosquito larva-bacterium interactions contribute to the evolution of immune system. This proposed project initiates a study on larval immune responses upon exposure to larvicidal bacterium Bacillus sphaericus and its impact on mosquito gut microbiota and malaria susceptibility. This pilot project will generate data towards comprehensive understanding the tripartite ecological interactions among malaria, gut microbiota and host immunity and its impact on vectorial capacity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Pilot Research Project (SC2)
Project #
5SC2GM092789-03
Application #
8432451
Study Section
Special Emphasis Panel (ZGM1-MBRS-X (GC))
Program Officer
Dunsmore, Sarah
Project Start
2011-03-01
Project End
2015-02-28
Budget Start
2013-03-01
Budget End
2015-02-28
Support Year
3
Fiscal Year
2013
Total Cost
$137,030
Indirect Cost
$40,530
Name
New Mexico State University Las Cruces
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
173851965
City
Las Cruces
State
NM
Country
United States
Zip Code
88003
Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2014) Insights from the genome annotation of Elizabethkingia anophelis from the malaria vector Anopheles gambiae. PLoS One 9:e97715
Kukutla, Phanidhar; Steritz, Matthew; Xu, Jiannong (2013) Depletion of ribosomal RNA for mosquito gut metagenomic RNA-seq. J Vis Exp :
Wang, Ying; Gilbreath 3rd, Thomas M; Kukutla, Phanidhar et al. (2011) Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya. PLoS One 6:e24767