Abstract

Malaria vector competence is largely determined by ecological interactions among mosquito gut microbiota, Plasmodium, and host immunity in the context of gut microenvironment. Mosquito immunity studies have been mainly focusing on the adults. Larva-pathogen interaction is an unignorable driving force in shaping mosquito immune system during the evolution. However, little is known about larva immune structure and its interaction with gut microbial flora. In this project we propose to use larvicidal Bacillus sphaericus to study mosquito larval immune response, and characterize larval immune architecture by using microarray based global expression profiling. The impact of larval immune activation on the dynamics of gut microbiota in both larvae and adults will be assessed by profiling gut bacterial community via high throughput pyrosequencing bacterial 16S rDNA marker. The influence of these parameters on the malaria susceptibility will also be evaluated. The project will generate data towards a better understanding of larval immunity and its impact on mosquito gut ecosystem.

Public Health Relevance

Mosquito larva-bacterium interactions contribute to the evolution of immune system. This proposed project initiates a study on larval immune responses upon exposure to larvicidal bacterium Bacillus sphaericus and its impact on mosquito gut microbiota and malaria susceptibility. This pilot project will generate data towards comprehensive understanding the tripartite ecological interactions among malaria, gut microbiota and host immunity and its impact on vectorial capacity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Pilot Research Project (SC2)
Project #
5SC2GM092789-03
Application #
8432451
Study Section
Special Emphasis Panel (ZGM1-MBRS-X (GC))
Program Officer
Dunsmore, Sarah
Project Start
2011-03-01
Project End
2015-02-28
Budget Start
2013-03-01
Budget End
2015-02-28
Support Year
3
Fiscal Year
2013
Total Cost
$137,030
Indirect Cost
$40,530

  Institution

Name
New Mexico State University Las Cruces
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
173851965
City
Las Cruces
State
NM
Country
United States
Zip Code
88003

  Publications

Krajacich, Benjamin J; Huestis, Diana L; Dao, Adama et al. (2018) Investigation of the seasonal microbiome of Anopheles coluzzii mosquitoes in Mali. PLoS One 13:e0194899
Champion, Cody J; Xu, Jiannong (2018) Redox state affects fecundity and insecticide susceptibility in Anopheles gambiae. Sci Rep 8:13054
Champion, Cody J; Xu, Jiannong (2017) The impact of metagenomic interplay on the mosquito redox homeostasis. Free Radic Biol Med 105:79-85
Pei, Dong; Jiang, Jinjin; Yu, Wanqin et al. (2015) The waaL gene mutation compromised the inhabitation of Enterobacter sp. Ag1 in the mosquito gut environment. Parasit Vectors 8:437
Wang, Yan; Yu, Wanqin; Shi, Hua et al. (2015) Historical survey of the kdr mutations in the populations of Anopheles sinensis in China in 1996-2014. Malar J 14:120
Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2014) Insights from the genome annotation of Elizabethkingia anophelis from the malaria vector Anopheles gambiae. PLoS One 9:e97715
An, Lingling; Pookhao, Nauromal; Jiang, Hongmei et al. (2014) Statistical approach of functional profiling for a microbial community. PLoS One 9:e106588
Kukutla, Phanidhar; Lindberg, Bo G; Pei, Dong et al. (2013) Draft Genome Sequences of Elizabethkingia anophelis Strains R26T and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae. Genome Announc 1:
Ma, Yajun; Xu, Jiannong; Yang, Zhenzhou et al. (2013) A video clip of the biting midge Culicoides anophelis ingesting blood from an engorged Anopheles mosquito in Hainan, China. Parasit Vectors 6:326
Kukutla, Phanidhar; Steritz, Matthew; Xu, Jiannong (2013) Depletion of ribosomal RNA for mosquito gut metagenomic RNA-seq. J Vis Exp :

Showing the most recent 10 out of 13 publications