Bacterial meningitis is a severe bacterial infection of the protective brain membranes and can become fatal in as early as 24 hours after symptoms are noticed. According to the World Health Organization, one million cases of bacterial meningitis are estimated to occur and 200,000 of these die annually. However, current approaches for meningitis diagnosis are either costly and time consuming (e.g. cell culture, which may take more than 48 hours.), or need expensive specialized equipment available only in laboratories (e.g. thermal cyclers needed for real-time PCR). All these factors limit the applications of those approaches in resource-limited settings. Considering that many cases of meningitis cases happened in rural high-poverty areas, and the high fatality rate of meningitis, a simple, low-cost, highly-sensitive device and method are greatly needed for immediate and early diagnosis of meningitis in resource-poor settings. The goal of this proposal is to develop a low-cost point of care (POC) device for rapid and highly-sensitive diagnosis of three different types of bacterial meningitis, namely Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumonia especially for resource-poor settings. DNA codes with different DNA sequences to target these pathogens will be grafted to paper by utilizing the interaction between the nanomaterial graphene oxide (GO) and single strand DNA. Multiple capture zones on microfluidic devices allow for the detection of these three different species of bacteria on chip simultaneously and specifically, based on DNA hybridization with specific DNA capture probes. A miniaturized film heater will be devised to integrate the loop-mediated isothermal amplification (LAMP) on chip, providing high detection sensitivity. In addition, we also aim to design and perfect an instrument-free, paper-based device for multiplex meningitis diagnosis in field. This project will provide a low-cost paper-based microfluidic POC device and method for immediate and early diagnosis of meningitis especially in resource-poor settings. It will also have great potential in the detection of various plant, animal, foodborne, and infectious diseases from other microorganisms.

Public Health Relevance

Bacterial meningitis is a severe bacterial infection of the protective brain membranes and can become fatal in as early as 24 hours after symptoms are noticed. Current approaches for meningitis diagnosis, however, are either costly and time consuming, or need expensive specialized equipment available only in laboratories, limiting the applications of those approaches in low-resource settings. We are developing a low-cost point of care (POC) biochip based on DNA testing for fast and early diagnosis of three different types of meningitis simultaneously especially for resource-poor settings.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Pilot Research Project (SC2)
Project #
1SC2GM105584-01
Application #
8475160
Study Section
Special Emphasis Panel (ZGM1-TWD-Y (SC))
Program Officer
Zlotnik, Hinda
Project Start
2013-05-01
Project End
2016-01-31
Budget Start
2013-05-01
Budget End
2014-01-31
Support Year
1
Fiscal Year
2013
Total Cost
$144,684
Indirect Cost
$44,684
Name
University of Texas El Paso
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
132051285
City
El Paso
State
TX
Country
United States
Zip Code
79968
Sanjay, Sharma T; Dou, Maowei; Fu, Guanglei et al. (2016) Controlled Drug Delivery Using Microdevices. Curr Pharm Biotechnol 17:772-87
Dou, Maowei; Lopez, Juan; Rios, Misael et al. (2016) A fully battery-powered inexpensive spectrophotometric system for high-sensitivity point-of-care analysis on a microfluidic chip. Analyst 141:3898-903
Fu, Guanglei; Sanjay, Sharma T; Dou, Maowei et al. (2016) Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer. Nanoscale 8:5422-7
Fu, Guanglei; Sanjay, Sharma T; Li, XiuJun (2016) Cost-effective and sensitive colorimetric immunosensing using an iron oxide-to-Prussian blue nanoparticle conversion strategy. Analyst 141:3883-9
Sanjay, Sharma T; Dou, Maowei; Sun, Jianjun et al. (2016) A paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers. Sci Rep 6:30474
Dou, Maowei; García Jr, José Mireles; Zhan, Sihui et al. (2016) Interfacial nano-biosensing in microfluidic droplets for high-sensitivity detection of low-solubility molecules. Chem Commun (Camb) 52:3470-3
Dou, Maowei; Sanjay, Sharma Timilsina; Benhabib, Merwan et al. (2015) Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms. Talanta 145:43-54
Sanjay, Sharma T; Fu, Guanglei; Dou, Maowei et al. (2015) Biomarker detection for disease diagnosis using cost-effective microfluidic platforms. Analyst 140:7062-81
Dou, Maowei; Dominguez, Delfina C; Li, XiuJun et al. (2014) A versatile PDMS/paper hybrid microfluidic platform for sensitive infectious disease diagnosis. Anal Chem 86:7978-86
Shen, Feng; Li, Xiujun; Li, Paul C H (2014) Study of flow behaviors on single-cell manipulation and shear stress reduction in microfluidic chips using computational fluid dynamics simulations. Biomicrofluidics 8:014109

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