From the formation of the female egg to the first three hours after fertilization, Drosophila development is controlled by maternal gene expression. During oogenesis, maternal gene products control of development of the oocyte and establish the body axes. Over the years many maternally-acting genes have been identified and characterized. The focus of this proposal is to study the role of one of these genes, lark, during oogenesis. Lark is an RNA-binding protein that has been shown to be important in organization of the actin cytoskeleton. To determine the mechanism of regulation we have identified 38 potential RNA targets, one of which, Dmoesin, is an actin-binding protein. Preliminary evidence shows that Dmoesin protein localization is regulated by Lark, likely through regulation of splicing in the nurse cell nuclei. The experimental design proposed in this application will address the validity of the other 37 potential RNA targets of Lark as well as determine the mechanism of Lark regulation of Dmoesin protein localization. These studies are important since although a lot has been learned about regulation of gene expression and localization in the developing egg chamber, little is known about the role of RNA splicing. These experiments should provide evidence suggesting RNA splicing plays an important role in the regulation of early development.

Public Health Relevance

The focus of this proposal is to investigate the mechanism by which the RNA-binding protein Lark regulates the actin cytoskeleton during oogenesis. Preliminary evidence suggests it functions as a splicing regulator. This research is important because little is known about the role of regulation of RNA splicing during oogenesis and results of the proposed experiments could begin to expand our knowledge of this potentially important mode of regulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Continuance Award (SC3)
Project #
5SC3GM089588-03
Application #
8206681
Study Section
Special Emphasis Panel (ZGM1-MBRS-7 (GC))
Program Officer
Krasnewich, Donna M
Project Start
2010-01-01
Project End
2013-12-31
Budget Start
2012-01-01
Budget End
2012-12-31
Support Year
3
Fiscal Year
2012
Total Cost
$120,965
Indirect Cost
$46,715
Name
York College
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
620128822
City
Jamaica
State
NY
Country
United States
Zip Code
11451
Huang, Yanmei; McNeil, Gerard P; Jackson, F Rob (2014) Translational regulation of the DOUBLETIME/CKIδ/ε kinase by LARK contributes to circadian period modulation. PLoS Genet 10:e1004536