The long-term objective of this research project is to develop circularly polarized luminescence (CPL) as a tool for enantioselective recognition of biomolecules and for investigating chiral structures in metal-containing biomolecular systems. Molecular chirality-the property whereby two mirror images of a molecule cannot be superimposed on each other-is crucial to modern drug research. While the difference between chiral structures may seem trivially small, the slight change in the compounds'three-dimensional structure profoundly alters the given compound's interaction with its surroundings. For example, in the 1960s, racemic thalidomide was widely used to treat morning sickness. One of the enantiomers was effective at reducing morning sickness, but unfortunately the drug's other enantiomer caused birth defects. For this reason, it is easy to understand why single-enantiomer drugs are attractive, and researchers are looking at them as possible treatments for cancer, cardiovascular disease, and central nervous system (CNS) defects. In 2009, estimates suggest that enantiopure drugs will produce $15 billion in revenue. The central hypothesis of this proposal is that CPL is an advancement over the common circular dichroism (CD) method due to its superiority in sensitivity, reliability, ease of use, and minimal sample preparation. Of special importance is that our methodological refinement of directly and selectively exciting the lanthanide(III) ion (Ln(III) = Eu, Tb) will increase discrimination between luminescent sites, making interpretation easier (i.e. CPL will reflect the time- averaged local helicity around the Ln(III) ion). Of special interest is the importance of using CPL for selectively studying only luminescent chromophores present in the systems of interest;CD, in contrast, is affected by most chromophores and/or equilibrium mixtures in an additive manner. More specifically, the study will (1) investigate the sensitivity and selectively of CPL spectroscopy used as an analytical tool for enantioselective recognition of biomolecules such as amino acids, (2) examine the effectiveness of CPL spectroscopy as a probe into the existence of chiral lanthanide structures and as an indicator of changes in the chiral structures, and (3) demonstrate the importance of using europium(III) CPL spectroscopy to understand the relationships between the chiral structures of proteins and their ability to bind metal ions (i.e. Ca(II), Mg(II)) where these metal ions are substituted by Eu(III) ions.

Public Health Relevance

With the knowledge that metal-binding proteins may account for as many as 40% of all proteins, this research field has gained a considerable interest and is still growing with the continuous discovery of new processes and functions of this class of proteins. Of special importance is to understand the function of the spectroscopically silent alkaline earth divalent cations Mg(II) and Ca(II) in many biological processes (i.e. enzyme activation, nucleic acid stabilization, muscle contraction, secretion, or synaptic transmission). On the other hand, the study of enantiomeric recognition of biological substrates is an ongoing active research because it can provide valuable information concerning molecular recognition mechanisms in biological materials.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Continuance Award (SC3)
Project #
5SC3GM089589-04
Application #
8399725
Study Section
Special Emphasis Panel (ZGM1-MBRS-X (CH))
Program Officer
Rogers, Michael E
Project Start
2010-01-01
Project End
2014-12-31
Budget Start
2013-01-01
Budget End
2014-12-31
Support Year
4
Fiscal Year
2013
Total Cost
$102,748
Indirect Cost
$31,097
Name
San Jose State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
056820715
City
San Jose
State
CA
Country
United States
Zip Code
95112
Nguyen, Brian T; Ingram, Andrew J; Muller, Gilles (2016) Localizing Perturbations of the Racemic Equilibria Involving Dipicolinate-Derived Lanthanide(III) Complexes. Chirality 28:325-31
Ray, César; Sánchez-Carnerero, Esther M; Moreno, Florencio et al. (2016) Bis(haloBODIPYs) with Labile Helicity: Valuable Simple Organic Molecules That Enable Circularly Polarized Luminescence. Chemistry 22:8805-8
Isla, Helena; Srebro-Hooper, Monika; Jean, Marion et al. (2016) Conformational changes and chiroptical switching of enantiopure bis-helicenic terpyridine upon Zn(2+) binding. Chem Commun (Camb) 52:5932-5
Feuillastre, Sophie; Pauton, Mathilde; Gao, Longhui et al. (2016) Design and Synthesis of New Circularly Polarized Thermally Activated Delayed Fluorescence Emitters. J Am Chem Soc 138:3990-3
Saleh, Nidal; Srebro, Monika; Reynaldo, Thibault et al. (2015) enantio-Enriched CPL-active helicene-bipyridine-rhenium complexes. Chem Commun (Camb) 51:3754-7
Saleh, Nidal; Moore 2nd, Barry; Srebro, Monika et al. (2015) Acid/base-triggered switching of circularly polarized luminescence and electronic circular dichroism in organic and organometallic helicenes. Chemistry 21:1673-81
Sánchez-Carnerero, Esther M; Agarrabeitia, Antonia R; Moreno, Florencio et al. (2015) Circularly Polarized Luminescence from Simple Organic Molecules. Chemistry 21:13488-500
Zhang, Xiao-Peng; Chang, Victoria Y; Liu, Jian et al. (2015) Potential switchable circularly polarized luminescence from chiral cyclometalated platinum(II) complexes. Inorg Chem 54:143-52
Sánchez-Carnerero, Esther M; Moreno, Florencio; Maroto, Beatriz L et al. (2014) Circularly polarized luminescence by visible-light absorption in a chiral O-BODIPY dye: unprecedented design of CPL organic molecules from achiral chromophores. J Am Chem Soc 136:3346-9
Shen, Chengshuo; Anger, Emmanuel; Srebro, Monika et al. (2014) Straightforward access to mono- and bis-cycloplatinated helicenes that display circularly polarized phosphorescence using crystallization resolution methods. Chem Sci 5:1915-1927

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