Effects of Retinoic Acid Signaling on Retinal Ganglion Cell Survival and Regeneration. Recovery of vision after optic nerve injury requires retinal ganglion cell (RGC) survival, axonal regrowth past the area of the lesion and reformation of appropriate synaptic targets. The adult mammalian visual system regenerates poorly, and past therapeutic efforts have enabled regrowth of only a small number of retinal axons. Frog RGCs, on the other hand, suffer an approximately 50% cell loss after injury, but regeneration and reconnection to target areas still occur because of the lack of inhibitory glial molecules and physical obstructions. This makes them a good model to explore which molecules influence RGC regeneration. Understanding what occurs after RGC injury is of great medical importance, because it results in blindness after optic nerve injury, and also in conditions such as glaucoma, diabetes and optic ischemia. The focus of the proposed work is to understand how the activity of retinoic acid (RA) signaling, known to exert effects on neurite outgrowth and homeostatic synaptic plasticity in the brain, affects the processes leading to recovery of vision after axotomy in the adult visual system.
The aims of this proposal are to (1) determine the role of RA and the intracellular signaling pathways it activates in promoting RGC survival after injury, (2) determine the role of RA promoting regeneration of RGC axons, and (3) investigate the effects of RA on the reformation of synaptic connections in the retinorecipient layer of the optic tectum.
The first aim will be accomplished by quantification of numbers of surviving RGCs, and Western blot analysis of regenerating retinal tissue.
The second aim will be addressed by measuring the number of viable retinal axons crossing the lesion area, changes in the speed of regeneration, and changes in the abundance of GAP43 (regeneration marker) and NAV-2 (responsive gene in RA-mediated neurite outgrowth) in non-treated optic nerves and nerves from animals treated with RA signaling agonists and antagonists. The effect of RA signaling on the activation of relevant intracellular signaling pathways in regenerating RGCs will also be studied.
The third aim will be carried out by quantifying anterogradely labeled retinal axons, the number of functional synaptic inputs, and the distribution of synaptic proteins in the retinorecipient layer f optic tectum after altering retinoic acid signaling and axotomy. Understanding the role of RA signaling in the response of retinal ganglion cells to nerve injury is important for the design of potential new therapeutic strategies.

Public Health Relevance

This research will help our understanding of the molecular pathways involved in the regrowth and reconnection of regenerating nerve cells in the central nervous system. This basic knowledge will increase our understanding of the molecular mechanisms involved in the formation and modulation of synaptic connections during regeneration. It will also have the potential to contribute towards improved therapeutic methods to promote recovery after injury and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Continuance Award (SC3)
Project #
5SC3GM116692-04
Application #
9624434
Study Section
Special Emphasis Panel (ZGM1)
Program Officer
Krasnewich, Donna M
Project Start
2016-02-01
Project End
2020-01-31
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
4
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Puerto Rico Med Sciences
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
948108063
City
San Juan
State
PR
Country
United States
Zip Code
00936
Duprey-Díaz, Mildred V; Blagburn, Jonathan M; Blanco, Rosa E (2016) Exogenous Modulation of Retinoic Acid Signaling Affects Adult RGC Survival in the Frog Visual System after Optic Nerve Injury. PLoS One 11:e0162626
Duprey-Díaz, Mildred V; Blagburn, Jonathan M; Blanco, Rosa E (2016) Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system. J Chem Neuroanat 77:80-92