This is a proposal for the renewal of a training grant, the primary purpose of which is to provide stipends for seven predoctoral and two postdoctoral fellows who will be broadly and intensively trained to conduct research on alcoholism. This program has a long history of training predoctoral and postdoctoral students in alcoholism research to help the national effort of producing the next generation of independent scientists with a focus on alcohol research. Training opportunities span the breadth of state-of-the-art approaches including molecular biology and genetics, electrophysiology, cellular imaging, neurochemistry, and behavior. Research models include both animal and human. The training program will promote and support collaborative research across multiple departmental units and Ph.D. programs including Clinical Psychology, Behavioral Neuroscience, Pharmacology, Neuroscience, and Cellular and Molecular Biology. Molecular biology training will encompass 1) studies of the function of excitatory and inhibitory ion channels in cellular expression systems (Mihic, Harris), and 2) development of new transgenic animal models (Harris, Mihic, Pierce-Shimomura, Atkinson), and 3) identification of ethanol responsive genes (Atkinson, Harris, Mihic, Pierce-Shimomura). Genetics training will involve genotyping of human and animal subjects (Fromme, Harris, Mayfield). Electrophysiological and microscopic imaging training will comprise experiments with whole cell and intracellular methods in single cells and in brain slices (Morrisett, Mihic, Harris, Atkinson, Pierce-Shimomura). Training in neurochemistry will focus on 1) intracellular and extracellular signaling mechanisms with an emphasis on phosphorylation (Morrisett, Mayfield), 2) release and transport of glutamate, dopamine, and other neurotransmitters (Gonzales, Harris), and 3) expression of proteins (Harris, Mayfield, Morrisett). Research on the behavioral effects of ethanol will include its influence on motor skills, reinforcement, anxiety, and withdrawal (Harris, Gonzales). Training in psychosocial aspects of alcohol research (Fromme) and the interaction of genotype and effects of alcohol drinking in humans (Fromme, Harris) will also be done. The training program will continue to emphasize professional development including improvement of oral and written communication skills. Predoctoral students will be required to complete a series of core course requirements, scientific ethics, experimental design, and statistical analysis, and grant writing, and will end up with a Ph.D. degree. Postdoctoral training will be for three years and consist of focused alcohol research guided by a faculty mentor. We will continue to focus on minority recruitment. The training faculty has an excellent history of collaboration with a primary focus on alcohol research. The research laboratories are well equipped with the latest instrumentation for neurochemical and behavioral testing.
This training program has direct relevance to public health, primarily in the area of alcoholism, a devastating disease in the United States and worldwide. Trainees will learn cutting edge research techniques and will perform research on alcohol related problems to provide new information that may guide treatments or further our understanding of the causes and consequences of the disease of alcoholism.
|Renteria, Rafael; Maier, Esther Y; Buske, Tavanna R et al. (2017) Selective alterations of NMDAR function and plasticity in D1 and D2 medium spiny neurons in the nucleus accumbens shell following chronic intermittent ethanol exposure. Neuropharmacology 112:164-171|
|Cofresí, Roberto U; Lewis, Suzanne M; Chaudhri, Nadia et al. (2017) Postretrieval Extinction Attenuates Alcohol Cue Reactivity in Rats. Alcohol Clin Exp Res 41:608-617|
|Reno, James M; Thakore, Neha; Cormack, Lawrence K et al. (2017) Negative Affect-Associated USV Acoustic Characteristics Predict Future Excessive Alcohol Drinking and Alcohol Avoidance in Male P and NP Rats. Alcohol Clin Exp Res 41:786-797|
|Robison, Christopher L; Martz, Julia R; Dominguez, Juan M (2017) Influence of preoptic estradiol on behavioral and neural response to cocaine in female Sprague-Dawley rats. Psychopharmacology (Berl) :|
|Wilhite, Emily R; Ashenhurst, James R; Marino, Elise N et al. (2017) Freshman year alcohol and marijuana use prospectively predict time to college graduation and subsequent adult roles and independence. J Am Coll Health :1-10|
|Park, Annie; Ghezzi, Alfredo; Wijesekera, Thilini P et al. (2017) Genetics and genomics of alcohol responses in Drosophila. Neuropharmacology 122:22-35|
|Tobiansky, Daniel J; Will, Ryan G; Lominac, Kevin D et al. (2016) Estradiol in the Preoptic Area Regulates the Dopaminergic Response to Cocaine in the Nucleus Accumbens. Neuropsychopharmacology 41:1897-906|
|Thakore, Neha; Reno, James M; Gonzales, Rueben A et al. (2016) Alcohol enhances unprovoked 22-28 kHz USVs and suppresses USV mean frequency in High Alcohol Drinking (HAD-1) male rats. Behav Brain Res 302:228-36|
|Farris, Sean P (2016) Moving Toward Understanding the Proteome Involved in Substance Abuse. Biol Psychiatry 79:422-4|
|Wolfe, Sarah A; Workman, Emily R; Heaney, Chelcie F et al. (2016) FMRP regulates an ethanol-dependent shift in GABABR function and expression with rapid antidepressant properties. Nat Commun 7:12867|
Showing the most recent 10 out of 147 publications