This is a proposal for the renewal of a training grant, the primary purpose of which is to provide stipends for seven predoctoral and two postdoctoral fellows who will be broadly and intensively trained to conduct research on alcoholism. This program has a long history of training predoctoral and postdoctoral students in alcoholism research to help the national effort of producing the next generation of independent scientists with a focus on alcohol research. Training opportunities span the breadth of state-of-the-art approaches including molecular biology and genetics, electrophysiology, cellular imaging, neurochemistry, and behavior. Research models include both animal and human. The training program will promote and support collaborative research across multiple departmental units and Ph.D. programs including Clinical Psychology, Behavioral Neuroscience, Pharmacology, Neuroscience, and Cellular and Molecular Biology. Molecular biology training will encompass 1) studies of the function of excitatory and inhibitory ion channels in cellular expression systems (Mihic, Harris), and 2) development of new transgenic animal models (Harris, Mihic, Pierce-Shimomura, Atkinson), and 3) identification of ethanol responsive genes (Atkinson, Harris, Mihic, Pierce-Shimomura). Genetics training will involve genotyping of human and animal subjects (Fromme, Harris, Mayfield). Electrophysiological and microscopic imaging training will comprise experiments with whole cell and intracellular methods in single cells and in brain slices (Morrisett, Mihic, Harris, Atkinson, Pierce-Shimomura). Training in neurochemistry will focus on 1) intracellular and extracellular signaling mechanisms with an emphasis on phosphorylation (Morrisett, Mayfield), 2) release and transport of glutamate, dopamine, and other neurotransmitters (Gonzales, Harris), and 3) expression of proteins (Harris, Mayfield, Morrisett). Research on the behavioral effects of ethanol will include its influence on motor skills, reinforcement, anxiety, and withdrawal (Harris, Gonzales). Training in psychosocial aspects of alcohol research (Fromme) and the interaction of genotype and effects of alcohol drinking in humans (Fromme, Harris) will also be done. The training program will continue to emphasize professional development including improvement of oral and written communication skills. Predoctoral students will be required to complete a series of core course requirements, scientific ethics, experimental design, and statistical analysis, and grant writing, and will end up with a Ph.D. degree. Postdoctoral training will be for three years and consist of focused alcohol research guided by a faculty mentor. We will continue to focus on minority recruitment. The training faculty has an excellent history of collaboration with a primary focus on alcohol research. The research laboratories are well equipped with the latest instrumentation for neurochemical and behavioral testing.
This training program has direct relevance to public health, primarily in the area of alcoholism, a devastating disease in the United States and worldwide. Trainees will learn cutting edge research techniques and will perform research on alcohol related problems to provide new information that may guide treatments or further our understanding of the causes and consequences of the disease of alcoholism.
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|Most, D; Ferguson, L; Blednov, Y et al. (2015) The synaptoneurosome transcriptome: a model for profiling the emolecular effects of alcohol. Pharmacogenomics J 15:177-88|
|Blednov, Yuri A; Benavidez, Jillian M; Black, Mendy et al. (2015) Peroxisome proliferator-activated receptors ? and ? are linked with alcohol consumption in mice and withdrawal and dependence in humans. Alcohol Clin Exp Res 39:136-45|
|Robinson, Gizelle; Most, Dana; Ferguson, Laura B et al. (2014) Neuroimmune pathways in alcohol consumption: evidence from behavioral and genetic studies in rodents and humans. Int Rev Neurobiol 118:13-39|
|Renteria, Rafael; Jeanes, Zachary M; Morrisett, Richard A (2014) Ethanol attenuation of long-term depression in the nucleus accumbens can be overcome by activation of TRPV1 receptors. Alcohol Clin Exp Res 38:2763-9|
|Ferguson, Laura B; Most, Dana; Blednov, Yuri A et al. (2014) PPAR agonists regulate brain gene expression: relationship to their effects on ethanol consumption. Neuropharmacology 86:397-407|
|Farris, Sean P; Mayfield, R Dayne (2014) RNA-Seq reveals novel transcriptional reorganization in human alcoholic brain. Int Rev Neurobiol 116:275-300|
|Reno, James M; Duvauchelle, Christine L (2014) Response to: making WAAVES in the vocalization community: how big is the splash? J Neurosci Methods 221:230-2|
|Reno, James M; Marker, Bryan; Cormack, Lawrence K et al. (2013) Automating ultrasonic vocalization analyses: the WAAVES program. J Neurosci Methods 219:155-61|
|Valenta, John P; Job, Martin O; Mangieri, Regina A et al. (2013) *-opioid receptors in the stimulation of mesolimbic dopamine activity by ethanol and morphine in Long-Evans rats: a delayed effect of ethanol. Psychopharmacology (Berl) 228:389-400|
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