This proposal is a competing renewal application for continuing support of a training program in the Molecular Biology of Eukaryotic Viruses, established at the University of California, Irvine (UCI) in 1988. The goal of this program is to train Ph.D. graduate students in the fundamental aspects of molecular virology as they relate to the regulation of gene expression, virus structure, virus-host interactions, and pathogenesis. During the past review period (9/07-8/13), ten predoctoral students were supported by this training program. An emphasis on research and training programs in the areas of viral modification of host cell functions, viruses requiring BSL3 containment, interdisciplinary research initiatives, and formal instruction in scientific writing is proposed for the next five yers. The number of faculty mentors for this program is 12, representing ten academic departments at UCI. Faculty members from these departments, along with those from other departments in the School of Biological Sciences and the School of Medicine, participate in the Cellular and Molecular Biosciences Graduate Program (CMB) at UCI, which oversees recruitment, admission, and first-year training of predoctoral students. All of the NIH Virology Training Grant trainees are part of the Virology Track in the CMB program. This track is comprised of faculty, students, postdoctoral fellows, and laboratory staff who have shared research interests in virology and related disciplines. The Virology training program includes elective courses in viral gene expression, molecular pathogenesis of viral infections, and scientific writing, as well as a Virology Journal Club. The research programs of faculty participants include the study of viral genome replication and transcription, RNA processing, viral translation and protein processing, assembly and transport of viral structural proteins, and the structures of virus particles. The virology faculty are also studying virus-host interactions that include alteration of host regulatoy molecules, growth control, cell cycle regulation, the mechanisms of integration of viral genomes into host cell DNA, and the subversion of host functions for virus gene expression. The viruses/viral systems being studied include murine leukemia virus, a sheep retrovirus (JSRV), HIV, yeast Ty3, poliovirus, human rhinovirus, coxsackievirus, Dengue virus, mouse hepatitis virus, SARS coronavirus, arenaviruses, herpes simplex virus, vaccinia virus, and DNA bacteriophages.

Public Health Relevance

The proposed program will train predoctoral students at the University of California, Irvine in a broad array of disciplines related to molecular virology. The program provides a research-based setting that includes formal coursework, seminar programs, research symposia, training in the handling of DNA and RNA viruses, training in the responsible conduct of research, and original laboratory research. This comprehensive training is intended to equip Ph.D. students with the necessary intellectual and research tools required for their future health-related careers in biotechnology, academia (including teaching), the pharmaceutical industry, and public health.

National Institute of Health (NIH)
Institutional National Research Service Award (T32)
Project #
Application #
Study Section
Microbiology and Infectious Diseases Research Committee (MID)
Program Officer
Robbins, Christiane M
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Irvine
Schools of Medicine
United States
Zip Code
Tsai, Becky Pinjou; Jimenez, Judith; Lim, Sharon et al. (2014) A novel Bcr-Abl-mTOR-eIF4A axis regulates IRES-mediated translation of LEF-1. Open Biol 4:140180
Angelini, Megan Mary; Neuman, Benjamin William; Buchmeier, Michael J (2014) Untangling membrane rearrangement in the nidovirales. DNA Cell Biol 33:122-7
Weinger, Jason G; Marro, Brett S; Hosking, Martin P et al. (2013) The chemokine receptor CXCR2 and coronavirus-induced neurologic disease. Virology 435:110-7
Clemens, Kristina; Bilanchone, Virginia; Beliakova-Bethell, Nadejda et al. (2013) Sequence requirements for localization and packaging of Ty3 retroelement RNA. Virus Res 171:319-31
Angelini, Megan M; Akhlaghpour, Marzieh; Neuman, Benjamin W et al. (2013) Severe acute respiratory syndrome coronavirus nonstructural proteins 3, 4, and 6 induce double-membrane vesicles. MBio 4:
Fitzgerald, Kerry D; Semler, Bert L (2013) Poliovirus infection induces the co-localization of cellular protein SRp20 with TIA-1, a cytoplasmic stress granule protein. Virus Res 176:223-31
Majumdar, Sudipta; Hajduczki, Agnes; Vithayathil, Rosemarie et al. (2011) In vitro evolution of ligands to the membrane protein caveolin. J Am Chem Soc 133:9855-62
Fitzgerald, Kerry D; Semler, Bert L (2009) Bridging IRES elements in mRNAs to the eukaryotic translation apparatus. Biochim Biophys Acta 1789:518-28
Beliakova-Bethell, Nadejda; Terry, Laura J; Bilanchone, Virginia et al. (2009) Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins. J Virol 83:11914-25
Houmani, Jennifer L; Davis, Christopher I; Ruf, Ingrid K (2009) Growth-promoting properties of Epstein-Barr virus EBER-1 RNA correlate with ribosomal protein L22 binding. J Virol 83:9844-53

Showing the most recent 10 out of 34 publications