This pre-doctoral training program at the Chemistry-Biology Interface involves 29 training faculty from three departments at the University of Minnesota: Chemistry, Medicinal Chemistry, and Biochemistry, Molecular Biology and Biophysics (BMBB). It seeks to provide a research training experience to its trainees that crosses the traditional disciplinary boundaries of Chemistry and Biology. Common research themes include: Biocatalysis and Biomolecular Design (Distefano, Kauzlauskus, Lipscomb, Ohlendorf, Schmidt-Dannert, Wackett, Wagner, Wilmot, York), Therapeutic Agents (Amin, Bernlohr, Distefano, Georg, Hoye, Mansky, Sturla, Tretyakova, Wagner, Xing), Chemistry of Disease (Arriaga, Bernlohr, Mansky, Murphy, Sturla, Tretyakova, Wagner, Walters, Xing), Metallobiochemistry (Amin, Lipscomb, Murphy, Ohlendorf, Pierre, Que, Tolman, Wackett, Wilmot, York), and Bioanalytical/Biophysical (Arriaga, Bowser, Griffin, Haynes, Pierre, Taton, Tretyakova, Thomas, Veglia, Walters). The research groups of the training faculty are all well supported and well equipped. There are extensive facilities for peptide and oligonucleotide synthesis, biofermentation, mass spectrometry, X-ray crystallography, NMR, and computation. Support is requested for 8 trainees each year of the program, each to be supported for a two-year period. Prospective trainees will generally have undergraduate degrees in chemistry, biochemistry or oiology. They will be expected to obtain thorough grounding in chemistry, as well as molecular biology and biochemistry. The particular emphasis of this training program is a focus on research problems that address synthetic/mechanistic questions in biological systems, with the goal of understanding and influencing key at the molecular level. The defining characteristic for this training program will be to allow first-rate students to grow into accomplished professionals both in their primary area of interest and in a complementary field. Students will choose from a menu of required coursework in chemistry and biology. In addition, laboratory rotations, a chemical biology colloquium, an annual symposium, journal clubs, and joint group meetings will enrich the graduate experience of the trainees.

Public Health Relevance

(Seeinstructions): The CBITG trainers and their trainees collectively carry out research on fundamental questions related to current important problems in public health. These include understanding how cancers are initiated in the cell, how obesity and diabetes develop and how a muscle works, designing and developing new drugs and assay probes against cancer, HIV, and Alzheimer's disease, and understanding how chemicals are broken down in the environment.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Institutional National Research Service Award (T32)
Project #
5T32GM008700-14
Application #
8280468
Study Section
Special Emphasis Panel (ZGM1-BRT-X (TG))
Program Officer
Fabian, Miles
Project Start
1999-07-01
Project End
2014-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
14
Fiscal Year
2012
Total Cost
$216,021
Indirect Cost
$12,735
Name
University of Minnesota Twin Cities
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Komor, Anna J; Rivard, Brent S; Fan, Ruixi et al. (2016) Mechanism for Six-Electron Aryl-N-Oxygenation by the Non-Heme Diiron Enzyme CmlI. J Am Chem Soc 138:7411-21
Palsuledesai, Charuta C; Ochocki, Joshua D; Kuhns, Michelle M et al. (2016) Metabolic Labeling with an Alkyne-modified Isoprenoid Analog Facilitates Imaging and Quantification of the Prenylome in Cells. ACS Chem Biol 11:2820-2828
Knoot, Cory J; Kovaleva, Elena G; Lipscomb, John D (2016) Crystal structure of CmlI, the arylamine oxygenase from the chloramphenicol biosynthetic pathway. J Biol Inorg Chem 21:589-603
Jasniewski, Andrew J; Knoot, Cory J; Lipscomb, John D et al. (2016) A Carboxylate Shift Regulates Dioxygen Activation by the Diiron Nonheme β-Hydroxylase CmlA upon Binding of a Substrate-Loaded Nonribosomal Peptide Synthetase. Biochemistry :
Dozier, Jonathan K; Distefano, Mark D (2015) Site-Specific PEGylation of Therapeutic Proteins. Int J Mol Sci 16:25831-64
Gee, Clifford T; Koleski, Edward J; Pomerantz, William C K (2015) Fragment screening and druggability assessment for the CBP/p300 KIX domain through protein-observed 19F NMR spectroscopy. Angew Chem Int Ed Engl 54:3735-9
Diaz-Rodriguez, Veronica; Ganusova, Elena; Rappe, Todd M et al. (2015) Synthesis of Peptides Containing C-Terminal Esters Using Trityl Side-Chain Anchoring: Applications to the Synthesis of C-Terminal Ester Analogs of the Saccharomyces cerevisiae Mating Pheromone a-Factor. J Org Chem 80:11266-74
Knoot, Cory J; Purpero, Vincent M; Lipscomb, John D (2015) Crystal structures of alkylperoxo and anhydride intermediates in an intradiol ring-cleaving dioxygenase. Proc Natl Acad Sci U S A 112:388-93
McCaffrey, Jesse E; James, Zachary M; Thomas, David D (2015) Optimization of bicelle lipid composition and temperature for EPR spectroscopy of aligned membranes. J Magn Reson 250:71-5
Manning, Benjamin M; Meyer, Audrey F; Gruba, Sarah M et al. (2015) Single-cell analysis of mast cell degranulation induced by airway smooth muscle-secreted chemokines. Biochim Biophys Acta 1850:1862-8

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