This project will address the need of INIA-West for validating the role of INIA candidate genes in excessive drinking. The RNA Interference Core will provide viral vectors for RNA interference-mediated knockdown or over-expression of candidate genes to INIA-West Investigators.
The specific aims of the project are to design short hairpin RNAs (shRNAs) targeting genes of interest and perform in vitro screening of shRNA efficacy, to produce virus expressing shRNAs for gene silencing in specific brain regions or in transgenic animals, to produce virus for over-expression of genes of interest, and to validate the effectiveness of gene silencing or over-expression in vivo. The viral tools developed by the INIA-West RNA Interference Core are crucial to understanding the role of specific genes in regulating excessive alcohol consumption.

Public Health Relevance

The goal of INIA-West is to identify the molecular, cellular, and behavioral changes that occur in specific brain regions that result in excessive alcohol consumption. The RNA Interference Core will provide support to INIA Investigators in reaching this goal by providing the tools necessary to study the role of genes in the brain that contribute to excessive drinking.

National Institute of Health (NIH)
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Research Project--Cooperative Agreements (U01)
Project #
Application #
Study Section
Special Emphasis Panel (ZAA1)
Program Officer
Reilly, Matthew
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Illinois at Chicago
Schools of Medicine
United States
Zip Code
Ding, Zheng-Ming; Ingraham, Cynthia M; Hauser, Sheketha R et al. (2017) Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Alcohol Clin Exp Res 41:1896-1906
Harris, R Adron; Bajo, Michal; Bell, Richard L et al. (2017) Genetic and Pharmacologic Manipulation of TLR4 Has Minimal Impact on Ethanol Consumption in Rodents. J Neurosci 37:1139-1155
Chen, H; He, D; Lasek, A W (2017) Midkine in the mouse ventral tegmental area limits ethanol intake and Ccl2 gene expression. Genes Brain Behav 16:699-708
Ji, Xincai; Saha, Sucharita; Gao, Guangping et al. (2017) The Sodium Channel ?4 Auxiliary Subunit Selectively Controls Long-Term Depression in Core Nucleus Accumbens Medium Spiny Neurons. Front Cell Neurosci 11:17
Haass-Koffler, C L; Henry, A T; Melkus, G et al. (2016) Defining the role of corticotropin releasing factor binding protein in alcohol consumption. Transl Psychiatry 6:e953
Lasek, Amy W (2016) Effects of Ethanol on Brain Extracellular Matrix: Implications for Alcohol Use Disorder. Alcohol Clin Exp Res 40:2030-2042
Schweitzer, Paul; Cates-Gatto, Chelsea; Varodayan, Florence P et al. (2016) Dependence-induced ethanol drinking and GABA neurotransmission are altered in Alk deficient mice. Neuropharmacology 107:1-8
Truitt, Jay M; Blednov, Yuri A; Benavidez, Jillian M et al. (2016) Inhibition of IKK? Reduces Ethanol Consumption in C57BL/6J Mice. eNeuro 3:
He, Donghong; Chen, Hu; Muramatsu, Hisako et al. (2015) Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain. J Neurochem 135:508-21
Chen, Hu; He, Donghong; Lasek, Amy W (2015) Repeated Binge Drinking Increases Perineuronal Nets in the Insular Cortex. Alcohol Clin Exp Res 39:1930-8

Showing the most recent 10 out of 24 publications