The Southern Division (SD) of the Cooperative Human Tissue Network (CHTN) at UAB has participated enthusiastically and effectively in all CHTN cooperative operations since its inception. The policies and guidelines that have been established have resulted in a dependable investigator source for a wide range of expertly processed and well-characterized human tissues, with associated histopathologic and demographic data, that continues to have a major impact on cancer research. With current advances in cancer research and technology, there has been an increased demand for more sophisticated tissue samples. To meet these demands, the SD proposes continuing its leading roles in:1) the design and modernization of the CHTN's computerized data management system to support increased networking of tissues and compatibility with caBIG;2) publicizing the availability of human tissues to the research community and promoting communication between the scientists receiving tissues and the CHTN;3) development of regulatory policies to include: positions on confidentiality and informed consent;inclusion of guidelines for implementation of new and modified Federal regulations and best practices for tissue resources;4) guidelines to protect against biohazards involved in use of human tissues;5) facilitating development of tissue resource infrastructure at multiple institutions through outreach and education;and 6) education of investigators regarding optimization of tissue requests. At the local level, the SD proposes to continue focusing on providing reliable high-quality human research tissues obtained under optimal conditions from surgical procedures and autopsies performed at UAB associated hospitals and subcontractor institutions. In response to investigator requests, the SD has already established these procedures and has allocated equipment and expertise to provide preneoplastic lesions, aliquots of tissues frozen within 15 minutes of recovery, as well as microdissection, tissue matrix arrays, mRNA/DNA samples, and clinical follow-up. In the current funding cycle (2001-2006), the SD, which primarily serves researchers in the Southern United States but also serves investigators throughout the US and Canada through networking, has provided over 35,000 fresh, frozen and fixed human tissue specimens to over 500 investigators. In the next funding cycle, consortium agreements with participating institutions will provide access to tissues from more than 150,000 surgical procedures with a goal of supplying 50,000 samples.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Project--Cooperative Agreements (U01)
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Application #
Study Section
Special Emphasis Panel (ZCA1-SRRB-K (M1))
Program Officer
Chuaqui, Rodrigo F
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University of Alabama Birmingham
Schools of Medicine
United States
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Atherton, Daniel S; Sexton, Katherine C; Otali, Dennis et al. (2016) Factors Affecting the Use of Human Tissues in Biomedical Research: Implications in the Design and Operation of a Biorepository. Methods Mol Biol 1381:1-38
Otali, D; Fredenburgh, J; Oelschlager, D K et al. (2016) A standard tissue as a control for histochemical and immunohistochemical staining. Biotech Histochem 91:309-26
Grizzle, William E; Gunter, Elaine W; Sexton, Katherine C et al. (2015) Quality management of biorepositories. Biopreserv Biobank 13:183-94
Bledsoe, Marianna J; Clayton, Ellen Wright; McGuire, Amy L et al. (2013) Return of research results from genomic biobanks: cost matters. Genet Med 15:103-5
Otali, Dennis; He, Qinghua; Grizzle, William E (2013) The effect of antigen retrieval on cells fixed in 10% neutral buffered formalin followed by transfer to 70% ethanol. PLoS One 8:e82405
Bledsoe, Marianna J; Grizzle, William E (2013) Use of human specimens in research: the evolving United States regulatory, policy, and scientific landscape. Diagn Histopathol (Oxf) 19:322-330
Otali, D; He, Q; Stockard, C R et al. (2013) Preservation of immunorecognition by transferring cells from 10% neutral buffered formalin to 70% ethanol. Biotech Histochem 88:170-80
Grizzle, William E (2012) A Critical Need for Increased Understanding of the Use of Human Tissues in Biomedical Research. J Cancer Sci Ther 4:16-17
Bledsoe, Marianna J; Grizzle, William E; Clark, Brian J et al. (2012) Practical implementation issues and challenges for biobanks in the return of individual research results. Genet Med 14:478-83
Grizzle, William E; Knoppers, Bartha M; Zeps, Nikolajs et al. (2011) What are the most oppressing legal and ethical issues facing biorepositories and what are some strategies to address them? Biopreserv Biobank 9:317-9

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