A multidisciplinary, four-year prospective study of the transmission and natural history of human herpes virus is proposed to (1) identify biologic and behavioral risk factors for acquisition of HHV-8/KSHV infection and (2) describe patterns for HHV- 8/KSHV virologic activity, types of host immunologic response, and the relationship of virologic activity and host response to the development of Kaposi's sarcoma (KS). The natural history of HHV-8/KSHV infection will be characterized in patients with and without HIV infection by (a) qualitative and quantitative longitudinal polymerase chain reaction measurement of HHV-8/KSHV in plasma, peripheral blood mononuclear cells, semen and saliva, (b) description of the evolution of anti-latency and anti-lytic phase associated antibodies to HHV-8/KSHV, (c) examination of host cytotoxic T-lymphocyte responses to HHV-8/KSHV at varying stages of infection, and (d) ascertainment of the temporal relationships between virologic activity, host responses, HIV infection, and the development of KS. Two existing cohorts of homosexual men, the San Francisco Men's Health Study and the San Francisco Young Men's Health Study, will be used to identify new seroconversions for antibodies to HHV-8/KSHV latency- associated nuclear antigens (LANA) and to obtain prospective samples of blood, semen, and saliva from men with pre-existing HHV-8/KSHV infection for varying duration, including men who have been seropositive since, at least, 1984. The researchers will follow 300 young men without prior evidence of HHV-8/KSHV infection, or whom 40 are HIV-infected, for the development of anti-LANA seroconversion. Between 35 and 40 incident infections are expected over the four years of follow-up. The natural history of infection will be observed in 200 men with pre-existing HHV- 8/KSHV infection; 100 of these are HIV co-infected.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01CA078124-04
Application #
6376773
Study Section
AIDS and Related Research Study Section 2 (ARRB)
Program Officer
Starks, Vaurice
Project Start
1998-06-01
Project End
2004-03-31
Budget Start
2001-04-01
Budget End
2004-03-31
Support Year
4
Fiscal Year
2001
Total Cost
$616,560
Indirect Cost
Name
University of California San Francisco
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Dollard, Sheila C; Butler, Lisa M; Jones, Alison M Graves et al. (2010) Substantial regional differences in human herpesvirus 8 seroprevalence in sub-Saharan Africa: insights on the origin of the ""Kaposi's sarcoma belt"". Int J Cancer 127:2395-401
Butler, Lisa M; Osmond, Dennis H; Jones, Alison Graves et al. (2009) Use of saliva as a lubricant in anal sexual practices among homosexual men. J Acquir Immune Defic Syndr 50:162-7
Phillips, Abigail M; Jones, Alison Graves; Osmond, Dennis H et al. (2008) Awareness of Kaposi's sarcoma-associated herpesvirus among men who have sex with men. Sex Transm Dis 35:1011-4
Chidzonga, Midion M; Mwale, Magda; Malvin, Kathy et al. (2008) Oral candidiasis as a marker of HIV disease progression among Zimbabwean women. J Acquir Immune Defic Syndr 47:579-84
Woodberry, Tonia; Suscovich, Todd J; Henry, Leah M et al. (2005) Impact of Kaposi sarcoma-associated herpesvirus (KSHV) burden and HIV coinfection on the detection of T cell responses to KSHV ORF73 and ORF65 proteins. J Infect Dis 192:622-9
Porco, Travis C; Martin, Jeffrey N; Page-Shafer, Kimberly A et al. (2004) Decline in HIV infectivity following the introduction of highly active antiretroviral therapy. AIDS 18:81-8
Martin, Jeffrey N (2003) Diagnosis and epidemiology of human herpesvirus 8 infection. Semin Hematol 40:133-42
Osmond, Dennis H; Buchbinder, Susan; Cheng, Amber et al. (2002) Prevalence of Kaposi sarcoma-associated herpesvirus infection in homosexual men at beginning of and during the HIV epidemic. JAMA 287:221-5
Brander, C; O'Connor, P; Suscovich, T et al. (2001) Definition of an optimal cytotoxic T lymphocyte epitope in the latently expressed Kaposi's sarcoma-associated herpesvirus kaposin protein. J Infect Dis 184:119-26
Martin, J N; Amad, Z; Cossen, C et al. (2000) Use of epidemiologically well-defined subjects and existing immunofluorescence assays to calibrate a new enzyme immunoassay for human herpesvirus 8 antibodies. J Clin Microbiol 38:696-701

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