? The development of the mammalian kidney is a very complex process, involving branching morphogenesis of the ureter and the formation of nepherons. Once the nepheron is formed, the next stage of development is for the loop of Henle and collecting duct to elongate into the renal pelvis, forming the medulla. Delineation of the spatial and temporal expression patterns of genes in kidney is essential for understanding its organogenesis and physiological function. Therefore, we proposed to analyze the expression patterns of 100 genes in the developing kidney. These genes are either novel or have not been reported to be expressed or to be functioning in the kidney. We will tag these genes with GFP to visualize the spatio-temporal expression patterns. Analyses of these mice will be enable us to: 1) determine the effect of the loss of the gene function on renal development; 2) follow the fate of the homozygous mutant cells as they are now marked by GFP; and 3) determine the subcellular localization of the gene product by visualizing the GFP tag, which often time is very revealing about a gene's function ? ?
| Chen, Ruoyu; Zhang, Qiao; Duan, Xiaoya et al. (2017) The 5-Hydroxymethylcytosine (5hmC) Reader UHRF2 Is Required for Normal Levels of 5hmC in Mouse Adult Brain and Spatial Learning and Memory. J Biol Chem 292:4533-4543 |
| York, J Philippe; Ren, Yi Athena; Zeng, Jie et al. (2016) Growth Arrest Specific 2 (GAS2) is a Critical Mediator of Germ Cell Cyst Breakdown and Folliculogenesis in Mice. Sci Rep 6:34956 |
| Alles, Melanie; Turchinovich, Gleb; Zhang, Pumin et al. (2014) Leukocyte ?7 integrin targeted by Krüppel-like factors. J Immunol 193:1737-46 |
| Chiu, Han Sheng; York, J Philippe; Wilkinson, Lorine et al. (2012) Production of a mouse line with a conditional Crim1 mutant allele. Genesis 50:711-6 |
