The Central Biochemistry Laboratory (CBL) serves the subjects and investigators of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD). The CBL provides participating clinical sites with protocols, kits, reagents, supplies, and transportation required for performing iohexol-based glomerular filtration rate (GFR) studies and collecting blood and urine for analysis at URMC and other laboratories. Blood analytes include electrolytes, BUN, creatinine, glucose, calcium, phosphorus, albumin, uric acid, lipid screen, hsCRP, intact PTH, and cystatin C. Urine analytes include creatinine, protein, and microalbumin. The CBL has determined optimal conditions for transporting these analytes, and provides ambient shippers for each visit and quarterly dry ice shippers for frozen specimens. The CBL also provides the kits and instructions for collection and shipment of blood, plasma, sera, urine, hair, and nail samples to NIH repositories. Biochemical data from each CKiD visit are reviewed and entered into Nephron, the CKiD data base. The CBL provides training at annual CKID meetings and is readily available to provide information, instruction, and support during CKiD visits, as needed by the site coordinators. The CBL contributes expertise in performing biochemical assays and clinical interpretation of the results. The CBL collaborates regularly with the Data Coordinating Center (DCC) to optimize GFR studies and to construct GFR estimating equations used during odd number visits at which no iohexol study is performed, maintain the Manual of Procedures, assure the quality control of all assays, and provide continuity of biochemical assays through changes in instrumentation. The CBL participates in all Steering Committee meetings and conference calls and collaborates with investigators in the generation of abstracts, presentations, and manuscripts, which further document the success of the CKiD study.
The aims of the CBL center on continuing to provide accurate, precise, and efficient measures of iohexol-based GFR and biochemical measurements of general kidney health. A pilot study to determine if fingerprick samples can replace serum collections for the iohexol GFR continues during this cycle. We plan to develop better GFR estimating equations, particularly for the children of Cohort 2, who have higher levels of GFR than those of Cohort 1. The CBL has submitted concept sheets to further determine if hyperuricemia predicts renal progression or hypertension, if microalbuminuria is a better predictor of renal progression than urinary protein, and to examine beta trace protein to determine if it is useful in predicting GFR. Finally, the CBL will continue to provide to the participating sites subject- and visit specific- laboratory kits for the collection, handling, and transport of samples, as well as receive, process, and analyze these samples on a daily basis, and provide timely data entry into Nephron. The long term goal is to better characterize the CKiD population biochemically, maintain the integrity of the data, and to optimize the collection of specimens in a manner that maintains recruitment and retention of CKiD subjects.

Public Health Relevance

The Central Biochemistry Laboratory of the continuing Prospective Study of Chronic Kidney Disease in Children (CKiD) provides participating clinical sites with protocols, subject- and visit- specific kits, reagents, supplies, and transportation required for performing kidney function tests and collecting blood and urine for analysis at URMC and other laboratories. These laboratory data are entered into the web-based database, Nephron. Also, the Central Lab provides training to coordinators and investigators, expertise to the CKiD study in regard to laboratory tests and the handling of blood, urine, and other human materials, and collaborations with the other CKiD investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project--Cooperative Agreements (U01)
Project #
2U01DK082194-06
Application #
8581911
Study Section
Special Emphasis Panel (ZDK1-GRB-9 (M3))
Program Officer
Moxey-Mims, Marva M
Project Start
2008-09-01
Project End
2018-07-31
Budget Start
2013-08-20
Budget End
2014-07-31
Support Year
6
Fiscal Year
2013
Total Cost
$350,000
Indirect Cost
$104,617
Name
University of Rochester
Department
Pediatrics
Type
Schools of Dentistry
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627
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Mitsnefes, Mark; Scherer, Philipp E; Friedman, Lisa Aronson et al. (2014) Ceramides and cardiac function in children with chronic kidney disease. Pediatr Nephrol 29:415-22
Nehus, Edward; Furth, Susan; Warady, Bradley et al. (2014) Correlates of leptin in children with chronic kidney disease. J Pediatr 165:825-9
Hartung, Erum A; Matheson, Matthew; Lande, Marc B et al. (2014) Neurocognition in children with autosomal recessive polycystic kidney disease in the CKiD cohort study. Pediatr Nephrol 29:1957-65
Kupferman, Juan C; Aronson Friedman, Lisa; Cox, Christopher et al. (2014) BP control and left ventricular hypertrophy regression in children with CKD. J Am Soc Nephrol 25:167-74
Margolick, Joseph B; Jacobson, Lisa P; Schwartz, George J et al. (2014) Factors affecting glomerular filtration rate, as measured by iohexol disappearance, in men with or at risk for HIV infection. PLoS One 9:e86311
Barletta, Gina-Marie; Flynn, Joseph; Mitsnefes, Mark et al. (2014) Heart rate and blood pressure variability in children with chronic kidney disease: a report from the CKiD study. Pediatr Nephrol 29:1059-65
Portale, Anthony A; Wolf, Myles; Juppner, Harald et al. (2014) Disordered FGF23 and mineral metabolism in children with CKD. Clin J Am Soc Nephrol 9:344-53
Akchurin, Oleh M; Schneider, Michael F; Mulqueen, Lucy et al. (2014) Medication adherence and growth in children with CKD. Clin J Am Soc Nephrol 9:1519-25

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